Larvicidal effects of a chitin-binding vicilin from Erythrina velutina seeds on the mediterranean fruit fly Ceratitis capitata

Journal of Agricultural and Food Chemistry
Leonardo L P MacedoMaurício P de Sales

Abstract

Chitin-binding vicilin from Erythrina velutina seeds was purified by ammonium sulfate followed by affinity chromatography on a chitin column and gel filtration on Superose-6-10-300-GL. The Erythrina velutina vicilin, called EvV, is a tetrameric glycoprotein composed of 1.85% carbohydrates and M r of 216.6 kDa, consisting of two subunits of M r of 54.8 and two subunits of M r of 50.8 kDa. The EvV homogeneity was confirmed in native PAGE where it was observed to be a unique acid-protein band with slow mobility in this gel. Effect of EvV on C. capitata larvae was examined by bioassay and its mechanism of action was determined by immunodetection techniques and fluorescence localization in chitin structures that are present in C. capitata digestory system. EvV when added to diet caused strong effect on mortality (ED50 of 0.14%) and larval mass (WD50 of 0.12%). These deleterious effects were associated to the binding to chitin structures present in peritrophic membrane and to gut epithelial cells, and its low digestibility in C. capitata digestive tract. These results are the first demonstration of a proteinaceous bioinsecticide from plant origin effective against C. capitata larvae. EvV may be part of the pest management programs or...Continue Reading

Citations

Jul 25, 2014·Parasitology Research·Patrícia Batista Barra Medeiros BarbosaMaria de Fátima de Freire Melo Ximenes
Jun 19, 2013·Plant Physiology and Biochemistry : PPB·Ana C B CruzElizeu A Santos
Sep 15, 2016·Molecular Nutrition & Food Research·Oliver SchlüterKarl-Heinz Engel
Oct 12, 2018·Archives of Insect Biochemistry and Physiology·Antonella RosaCarla Masala
Nov 17, 2019·Food Research International·Dele RaheemConrado Carrascosa
Sep 2, 2021·Saudi Journal of Biological Sciences·A F J FrançaA F Uchôa

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