Laryngeal abductor muscle reinnervation in a pig model

Acta Oto-laryngologica
Martin A BirchallGiorgio Terenghi

Abstract

To develop a large animal model for studies of laryngeal abductor reinnervation. Six minipigs underwent unilateral anastomosis of the phrenic nerve-abductor branch of the recurrent laryngeal nerve (RLN). Polyhydroxybutyrate (PHB) conduits were used for repair. At each of 30, 60 and 120 days, 2 animals underwent video laryngeal endoscopy (VLE) and were then killed. VLE was also performed in the 120-day pair at 60 days. Nerve-conduit-nerve-muscle samples were fixed for light and immunofluorescence (pan-neurofilaments, S-100) microscopy. Laryngeal muscles were harvested (myosin heavy chain analysis). VLE showed recovery of abductor function in 1 animal at 60 days and in 1 at 120 days. Haematoxylin-eosin staining demonstrated a complex inflammatory response. Eosinophil recruitment was observed. Stepwise regeneration and reorganization of the distal nerve between 30 and 120 days was observed with pan-NF staining. The mean minimum diameter in the reinnervated posterior crico-arytenoids tended to increase for up to 120 days. Anastomosis of the phrenic nerve-abductor branch of the RLN with a PHB conduit in a pig can result in functional and histological recovery within 2-4 months and appears to at least sustain abductor muscle fibre mo...Continue Reading

References

Jul 1, 1989·The Annals of Otology, Rhinology, and Laryngology·J P MarieJ Senant
Apr 1, 1997·Archives of Otolaryngology--head & Neck Surgery·J T van Lith-BijlH F Mahieu
Jul 1, 1997·The European Journal of Neuroscience·G D SterneG Terenghi
Apr 25, 2000·The Annals of Otology, Rhinology, and Laryngology·R L Crumley
Mar 29, 2001·The Journal of Laryngology and Otology·P Stavroulaki, M Birchall

❮ Previous
Next ❯

Citations

Sep 16, 2010·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M A BirchallP Macchiarini
Sep 29, 2006·Journal of Anatomy·Paul J Kingham, Giorgio Terenghi
Dec 22, 2005·Clinical and Experimental Immunology·E BarkerM Bailey
Feb 8, 2007·The European Journal of Neuroscience·Paul J KinghamMartin A Birchall
Apr 24, 2008·Transplantation·Martin Birchall, Paolo Macchiarini
Jun 16, 2010·Annals of Plastic Surgery·Thomas ScholzGregory R D Evans
Jul 12, 2005·Muscle & Nerve·Paul J KinghamGiorgio Terenghi
Mar 1, 2019·Muscle & Nerve·Marta CerconeJonathan Cheetham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Archives of Otolaryngology--head & Neck Surgery
J T van Lith-BijlH F Mahieu
Archives of Otolaryngology--head & Neck Surgery
J T van Lith-BijlH F Mahieu
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery
Xiaoxia QiuSiwen Xia
Indian Journal of Otolaryngology and Head and Neck Surgery : Official Publication of the Association of Otolaryngologists of India
J T van Lith-Bijl, H F Mahieu
© 2021 Meta ULC. All rights reserved