LasΔ5315 Effector Induces Extreme Starch Accumulation and Chlorosis as Ca . Liberibacter asiaticus Infection in Nicotiana benthamiana

Frontiers in Plant Science
Marco PitinoYongping Duan

Abstract

Huanglongbing (HLB), a destructive plant bacterial disease, severely impedes worldwide citrus production. HLB is associated with a phloem-limited α-proteobacterium, Candidatus Liberibacter asiaticus (Las). Las infection causes yellow shoots and blotchy mottle on leaves and is associated with excessive starch accumulation. However, the mechanisms underlying the starch accumulation remain unknown. We previously showed that the Las5315mp effector induced callose deposition and cell death in Nicotiana benthamiana. In this study, we demonstrated that Las can experimentally infect N. benthamiana via dodder transmission. Furthermore, we revealed another key function of the Las5315 effector by demonstrating that transient expression of the truncated form of the effector, LasΔ5315, induced excessive starch accumulation by 6 fold after 8 dpi in N. benthamiana after removal of the chloroplast transit peptide from the Las5315mp. The induction mechanisms of LasΔ5315 in N. benthamiana were attributed to the up-regulation of ADP-glucose pyrophosphorylase, granule-bound starch synthase, soluble starch synthase, and starch branching enzyme for increasing starch production, and to the significant down-regulation of the starch degradation enzymes...Continue Reading

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Citations

Dec 28, 2019·Pathogens·Matthias KretschmerJames Kronstad
Oct 31, 2020·International Journal of Molecular Sciences·Yinghui ZhouHuasong Zou
Sep 29, 2020·Molecular Plant-microbe Interactions : MPMI·Kelley J ClarkWenbo Ma
Jan 14, 2022·Phytopathology·Agustina De FrancescoWenbo Ma

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Methods Mentioned

BETA
transgenic
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