Laser ablation-combustion-GC-IRMS--a new method for online analysis of intra-annual variation of delta13C in tree rings

Tree Physiology
Brigit SchulzeErnst-Detlef Schulze

Abstract

We present a new, rapid method for high-resolution online determination of delta13C in tree rings, combining laser ablation (LA), combustion (C), gas chromatography (GC) and isotope ratio mass spectrometry (IRMS) (LA-C-GC-IRMS). Sample material was extracted every 6 min with a UV-laser from a tree core, leaving 40-microm-wide holes. Ablated wood dust was combusted to CO2 at 700 degrees C, separated from other gases on a GC column and injected into an isotope ratio mass spectrometer after removal of water vapor. The measurements were calibrated against an internal and an external standard. The tree core remained intact and could be used for subsequent dendrochronological and dendrochemical analyses. Cores from two Scots pine trees (Pinus sylvestris spp. sibirica Lebed.) from central Siberia were sampled. Inter- and intra-annual patterns of delta13C in whole-wood and lignin-extracted cores were indistinguishable apart from a constant offset, suggesting that lignin extraction is unnecessary for our method. Comparison with the conventional method (microtome slicing, elemental analysis and IRMS) indicated high accuracy of the LA-C-GC-IRMS measurements. Patterns of delta13C along three parallel ablation lines on the same core showed ...Continue Reading

Citations

Apr 9, 2010·Plant, Cell & Environment·Britta EilmannAndreas Rigling
Jul 16, 2013·Plant, Cell & Environment·Giovanna BattipagliaPaolo Cherubini
Jul 7, 2015·Trees : Structure and Function·Peter HietzMichael Grabner
Feb 26, 2010·Isotopes in Environmental and Health Studies·Willi A Brand
Apr 14, 2011·Rapid Communications in Mass Spectrometry : RCM·James J MoranHelen W Kreuzer
Jul 27, 2005·Phytochemical Analysis : PCA
Jun 21, 2016·Rapid Communications in Mass Spectrometry : RCM·M Isaac-RentonK Treydte
Jan 1, 2009·Functional Plant Biology : FPB·David M DrewGeoffrey M Downes
Dec 6, 2020·The Science of the Total Environment·Gilberto BindaDamiano Monticelli

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