Laser Ablation Remote-Electrospray Ionisation Mass Spectrometry (LARESI MSI) Imaging-New Method for Detection and Spatial Localization of Metabolites and Mycotoxins Produced by Moulds.

Toxins
Justyna Szulc, Tomasz Ruman

Abstract

To date, no method has been developed to assess the distribution of mycotoxins on the surface of grains, or other plant material, and the depth of their penetration into the interior. The Infrared (IR) Laser Ablation-Remote-Electrospray Ionization (LARESI) platform coupled to a tandem mass spectrometer (MS/MS), measuring in selected reaction monitoring (SRM) mode, was employed for the targeted imaging of selected metabolites of Aspergillus fumigatus, including mycotoxins in biological objects for the first time. This methodology allowed for the localisation of grain metabolites and fungal metabolites of grain infected by this mould. The distribution of metabolites in spelt grain was differentiated: fumigaclavine C, fumitremorgin C, and fumiquinazoline D were located mainly in the embryo, brevianamide F in the seed coat, and fumagillin in the endosperm. The LARESI mass spectrometry imaging method can be used in the future for the metabolomic analysis of mould metabolites in various plants and agricultural products.

References

Aug 1, 1985·Applied and Environmental Microbiology·H K AbbasD J Pusch
Jan 1, 1994·Food Additives and Contaminants·E W SydenhamP G Thiel
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·J Lippincott-SchwartzA Kenworthy
Sep 25, 2001·Toxicology·H S Hussein, J M Brasel
Apr 2, 2002·Advances in Experimental Medicine and Biology·Douglas L Park
Jul 15, 2003·Clinical Microbiology Reviews·J W Bennett, M Klich
Jan 17, 2004·Food Additives and Contaminants·K A ScudamoreS J MacDonald
Aug 18, 2005·Rapid Communications in Mass Spectrometry : RCM·Alexander K MullenKate R Sharples
May 16, 2007·Analytical Chemistry·Klaus DreisewerdGottfried Pohlentz
Sep 7, 2007·International Journal of Food Microbiology·Lloyd B Bullerman, Andreia Bianchini
May 13, 2008·Journal of the American Society for Mass Spectrometry·Erin H SeeleyRichard M Caprioli
Oct 24, 2008·Phytopathology·David G SchmaleGary C Bergstrom
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M Rivas CasadoS Origgi
Mar 28, 2012·The Plant Journal : for Cell and Molecular Biology·Young Jin LeeBasil J Nikolau
Jan 17, 2013·Analytical Chemistry·Joanna NiziołTomasz Ruman
Apr 30, 2013·Frontiers in Plant Science·Andrea Matros, Hans-Peter Mock
Jan 2, 2016·Current Opinion in Biotechnology·Laura AnfossiClaudio Baggiani
Feb 24, 2016·Frontiers in Plant Science·Yonghui DongPietro Franceschi
Feb 8, 2018·Journal of the Science of Food and Agriculture·Hina ShanakhatSilvana Cavella
Oct 19, 2019·Journal of Bioscience and Bioengineering·Adinda P WismanShuichi Shimma

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