Laser-assisted 3D bioprinting of exocrine pancreas spheroid models for cancer initiation study.

Biofabrication
Davit HakobyanHugo Oliveira

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is the most common malignancy of the pancreas. It has shown a poor prognosis and a rising incidence in the developed world. Other pathologies associated with this tissue include pancreatitis, a risk condition for pancreatic cancer. The onset of both pancreatitis and pancreatic cancer follows a common pattern: exocrine pancreatic acinar cells undergo a transdifferentiation to duct cells that triggers a 3D restructuration of the pancreatic tissue. However, the exact mechanism underlying this process remains partially undefined. Further understanding the cellular events leading to PDAC could open new avenues in the development of novel therapeutic approaches. Since current 2D cell culture models fail to mimic the tridimensional complexity of the pancreatic tissue, new in vitro models are urgently needed. Here, we generated 3D pancreatic cell spheroid arrays using laser-assisted bioprinting and characterized their phenotypic evolution over time through image analysis and phenotypic characterization. We show that these bioprinted spheroids, composed of both acinar and ductal cells, can replicate the initial stages of PDAC development. This bioprinted miniaturized spheroid-based array model sho...Continue Reading

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Citations

Oct 3, 2020·Alternatives to Laboratory Animals : ATLA
Aug 19, 2020·Sensors·Mohamed SharafeldinJames F Rusling
Jan 26, 2021·Cellular Oncology (Dordrecht)·Axel BengtssonDaniel Ansari
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Mar 27, 2021·Biomedical Materials·Sathyan Vivekanand Anandhan, Uma Maheswari Krishnan
Jul 6, 2021·Essays in Biochemistry·Seunggyu JeonHyun-Wook Kang

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