Laser-assisted hatching increases pregnancy and implantation rates in cryopreserved embryos that were allowed to cleave in vitro after thawing: a prospective randomized study

Human Reproduction
Basak BalabanAycan Isiklar

Abstract

Cryopreservation of embryos may lead to zona hardening that may compromise in vivo hatching and implantation following thawing and transfer. Assisted hatching (AH) has been advocated as a means of assisting the natural hatching process and enhancing implantation. The aim of this study was to assess in a prospective randomized manner the effect of laser-assisted hatching (LAH) on implantation as well as clinical and multiple pregnancy rates (the primary outcome) after the transfer of frozen-thawed embryos. All embryos were thawed the day before transfer, and LAH was performed the next day on embryos that cleaved. Control group consisted of embryos that were transferred without AH. The performance of LAH significantly increased implantation (9.9 versus 20.1%, P < 0.01), clinical pregnancy (27.3 versus 40.9, P < 0.05) and multiple pregnancy rates (16 versus 40.3%, P < 0.07). In the LAH group, significantly more excess embryos that were left in culture hatched in vitro. LAH improves the outcome of frozen-thawed embryo transfer when performed before transfer on embryos that were allowed to cleave.

References

Aug 1, 1991·Journal of in Vitro Fertilization and Embryo Transfer : IVF·J Cohen
Aug 1, 1986·Fertility and Sterility·J TestartR Frydman
Jan 1, 1995·Journal of Assisted Reproduction and Genetics·M C SchieweJ P Balmaceda
Feb 1, 1997·Journal of Assisted Reproduction and Genetics·J Tao, R Tamis
Jun 1, 1997·Human Reproduction·P O KarlströmM Wikland
May 1, 2002·Human Reproduction·Basak BalabanAycan Isiklar
Sep 2, 2003·Journal of Assisted Reproduction and Genetics·Hassan N SallamAbdel Fattah Agameya

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Citations

Aug 9, 2008·Journal of Assisted Reproduction and Genetics·Rouhollah FathiPoopak Eftekhari-Yazdi
Oct 16, 2009·Journal of Assisted Reproduction and Genetics·Kenichiro HiraokaKazuo Kinutani
Jul 5, 2013·Journal of Assisted Reproduction and Genetics·Daniel J KaserCatherine Racowsky
Apr 9, 2011·Human Reproduction Update·Wellington P MartinsCarolina O Nastri
Mar 21, 2013·Reproductive Biology and Endocrinology : RB&E·Chanchai ChailertTeraporn Vutyavanich
Sep 9, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Yi-Fan GuGuang-Xiu Lu
Oct 18, 2008·Human Fertility : Journal of the British Fertility Society·Ingrid GranneUNKNOWN British Fertility Society
Jul 12, 2007·Reproductive Biomedicine Online·Kenichiro HiraokaKazuo Kinutani
Feb 2, 2010·Reproductive Biomedicine Online·Mojtaba Rezazadeh ValojerdiBahar Movaghar
Apr 7, 2009·Gynécologie, obstétrique & fertilité·J Blessmann-RosetB Macé
Dec 3, 2014·Reproductive Biomedicine Online·Hanying ZhouWenhao Shi
Jan 20, 2018·Journal of Assisted Reproduction and Genetics·Alessandra AlteriPatrizia Rubino
Dec 14, 2012·The Cochrane Database of Systematic Reviews·Sarah-Kate CarneyLinsey Nelson
Nov 5, 2015·Biochemistry. Biokhimii︠a︡·E O ZakharchenkoV A Nadtochenko
Mar 18, 2021·The Cochrane Database of Systematic Reviews·Lauren LaceyM Ahsan Akhtar

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