Laser-assisted microdissection of the zona pellucida facilitates polar body biopsy

Fertility and Sterility
M MontagHans van der Ven

Abstract

To investigate whether polar body biopsy can be performed after laser microdissection of the zona pellucida (ZP). Mouse zygotes were allocated randomly to three groups. The zygotes were subjected to laser microdissection of the ZP and polar body biopsy (group 1), laser microdissection alone (group 2), or no treatment (group 3). University-based IVF program. Animal study. A hole was drilled in the ZP of mouse zygotes using a 1.48-micron noncontact diode laser. A microneedle was inserted and the polar body was aspirated. The efficacy of polar body biopsy after laser microdissection of the ZP was evaluated. The laser diode beam allowed for precise drilling of a 14- to 18-micron hole in the ZP. Polar bodies could be aspirated without damaging the zygote and did not disintegrate during the biopsy. Zygotes developed to blastocysts and underwent the same hatching as control zygotes. Lower hatching rates were observed in untreated zygotes. Laser microdissection of the ZP with a noncontact laser system facilitates subsequent polar body biopsy. The use of blunt-ended micropipettes greatly reduces the risk of damage to the zygote or the polar body. This procedure makes polar body biopsy more accurate and effective for preimplantation gene...Continue Reading

References

Oct 1, 1996·Human Reproduction·B Reubinoff, A Shushan

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Citations

Sep 18, 2001·Prenatal Diagnosis·A De Vos, A Van Steirteghem
Dec 18, 2013·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·M MontagT Strowitzki
May 25, 2010·Journal of Assisted Reproduction and Genetics·Ibrahim HammoudFrancois Vialard
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Oct 11, 2005·Fertility and Sterility·Vladimir IsachenkoHans van der Ven
May 29, 2001·Journal of Biomedical Optics·D H Douglas-Hamilton, J Conia
Sep 30, 2005·Prenatal Diagnosis·Markus MontagHans van der Ven
May 17, 2000·Current Opinion in Obstetrics & Gynecology·J C Harper, J D Delhanty
Sep 10, 2009·Deutsches Ärzteblatt International·Georg GriesingerKlaus Diedrich

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