Laser Cleavable Probes-Based Cell Surface Engineering for in Situ Sialoglycoconjugates Profiling by Laser Desorption/Ionization Mass Spectrometry

Analytical Chemistry
Jie SunZongxiu Nie

Abstract

Cell-surface sialoglycoconjugates (sialoglycoproteins and sialoglycolipids) play important roles in cell-cell interactions and related tumor metastasis process. Although there have been some analytical methods to evaluate the sialoglycoconjugates, an effective method providing both qualitative and quantitative information is still deficient. Here we establish an extraction-free, sensitive, and high-throughput platform to realize in situ detection of the cell-surface sialoglycoconjugates on various cell lines, e.g., cancer and normal cells by laser desorption/ionization mass spectrometry (LDI MS). In this proposal, azide groups were introduced into the ends of cell-surface sialoglycoconjugates by the biorthogonal method, and then the sialoglycoconjugates were armed with a laser-cleavable probe (Tphsene) through click chemistry. We can easily get the probes signal under laser irradiation, which reflected the presence of cell-surface sialoglycoconjugates. Different cell lines were discriminated simultaneously, and the LDI relative quantification agreed with fluorescent results. Besides, a linear quantitation relationship in the range of 100 fmol to 100 pmol was obtained with a designed and synthesized internal standard (phTsane) a...Continue Reading

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Citations

Oct 30, 2019·Journal of the American Society for Mass Spectrometry·Hani Nasser Abdelhamid, Hui-Fen Wu
Apr 20, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Anuja Shreeram KulkarniKun Qian
Aug 27, 2021·Analytical and Bioanalytical Chemistry·Shuting XuHuwei Liu
Sep 25, 2021·Analytical Chemistry·Jie SunMichael L Gross

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