Laser Desorption/Ionisation Mass Spectrometry Imaging of European Yew (Taxus baccata) on Gold Nanoparticle-enhanced Target

Phytochemical Analysis : PCA
Adrian Arendowski, Tomasz Ruman

Abstract

European yew (Taxus baccata) is a plant known to man for centuries as it produces many interesting and important metabolites. These chemical compounds were repeatedly analysed by various analytical techniques, but none of the methods used so far allowed the localisation of the chemical compounds within the tissue and also correlation between plant morphology and its biochemistry. Visualisation of the spatial distribution of yew metabolites with nanoparticle-based mass spectrometry imaging. Compounds occurring on cross-section of a one-year yew sprig has been transferred to gold nanoparticle-enhanced target (AuNPET) by imprinting. The imprint was then subjected to mass spectrometry imaging analysis. Nanoparticle-enhanced mass spectrometry imaging made it possible to study the distribution of selected compounds in the European yew tissue, including taxanes - terpene alkaloids characteristic for the Taxus genus. Results prove that aspartate, taxinine M, baccatin IV and taxine B are located mainly in the cortex. Taxuspine W was located in the vascular tissue. Maleate was found to be located mainly in the phloem tissue. In contrast, the proton adduct of chlorophyll b was found in the external layer of twigs. The results presented a ...Continue Reading

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Citations

Feb 10, 2018·Phytochemical Analysis : PCA·Lydia Siegle, Jörg Pietsch
Aug 25, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Adrian ArendowskiTomasz Ruman

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