Laser direct-write of single microbeads into spatially-ordered patterns

Biofabrication
Theresa B PhamduyDouglas B Chrisey

Abstract

Fabrication of heterogeneous microbead patterns on a bead-by-bead basis promotes new opportunities for sensors, lab-on-a-chip technology and cell-culturing systems within the context of customizable constructs. Laser direct-write (LDW) was utilized to target and deposit solid polystyrene and stem cell-laden alginate hydrogel beads into computer-programmed patterns. We successfully demonstrated single-bead printing resolution and fabricated spatially-ordered patterns of microbeads. The probability of successful microbead transfer from the ribbon surface increased from 0 to 80% with decreasing diameter of 600 to 45 µm, respectively. Direct-written microbeads retained spatial pattern registry, even after 10 min of ultrasonication treatment. SEM imaging confirmed immobilization of microbeads. Viability of cells encapsulated in transferred hydrogel microbeads achieved 37 ± 11% immediately after the transfer process, whereas randomly-patterned pipetted control beads achieved a viability of 51 ± 25%. Individual placement of >10 µm diameter microbeads onto planar surfaces has previously been unattainable. We have demonstrated LDW as a valuable tool for the patterning of single, micrometer-diameter beads into spatially-ordered patterns.

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Citations

Jan 31, 2014·The Analyst·Valentin RomanovBenjamin D Brooks
Apr 15, 2014·Trends in Biotechnology·Peter ErtlGünter Lepperdinger
Dec 25, 2012·Trends in Biotechnology·Savas Tasoglu, Utkan Demirci
Mar 1, 2016·Journal of Cellular Physiology·Hope E BurksWalter L Murfee
Jul 21, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Theresa B PhamduyDouglas B Chrisey
Nov 23, 2012·Biofabrication·Jingyuan YanDouglas B Chrisey
Mar 1, 2016·Biofabrication·J L CurleyD B Chrisey
Dec 13, 2019·Biofabrication·A MarquezC Molpeceres
May 21, 2020·Materials·Fei XingUlrike Ritz
Jul 4, 2017·Biomicrofluidics·Zhengyi ZhangYong Huang
Nov 22, 2016·Annals of the New York Academy of Sciences·Brad J TricomiDavid T Corr

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