Laser-induced breakdown spectroscopy (LIBS) as a novel technique for detecting bacterial infection in insects

Scientific Reports
Nabil KillinyLuis Ponce Cabrera

Abstract

To prevent the spread of diseases in humans, animals or plants, determining whether potential vectors are infected is crucial. For example, early detection of the citrus disease Huanglongbing, which has been a scourge on the citrus industries around the world, is a critical need. This vector-borne disease is transmitted by Diaphorina citri, the Asian citrus psyllid, which carries the putative bacterial phytopathogen, Candidatus Liberibacter asiaticus (CLas). In this investigation, we introduced Laser-Induced Breakdown Spectroscopy (LIBS) to reveal key biochemical differences between CLas-infected and non-infected psyllids. The emission spectra captured from laser ablation of CLas-infected and healthy psyllids were processed through the principal component analysis (PCA) method and compared. Thirteen peaks from seven different elements were detected in D. citri. The t-test showed that CLas-infected D. citri were deficients in zinc, iron, copper, magnesium, calcium, and nitrogen. The PCA showed that LIBS can successfully differentiate between CLas-infected and healthy D. citri by comparing their elemental profile. In this work, we demonstrated a method that allows for a fast and precise compositional microanalysis of an insect ve...Continue Reading

References

Jul 1, 1994·International Journal of Systematic Bacteriology·S JagoueixM Garnier
Apr 27, 2010·Annual Review of Phytopathology·Tim R Gottwald
Feb 14, 2012·Plant Physiology and Biochemistry : PPB·Juan M Cevallos-CevallosJosé I Reyes-De-Corcuera
Jan 16, 2013·Annual Review of Entomology·Elizabeth E Grafton-CardwellPhilip A Stansly
Feb 28, 2013·Phytopathology·Nian Wang, Pankaj Trivedi
Jun 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jo Ann LeeBurton H Singer
Oct 16, 2015·Journal of Integrative Plant Biology·John V da GraçaHongwei Zhao
Feb 15, 2017·Archives of Insect Biochemistry and Physiology·Abdelaziz KishkNabil Killiny
Apr 28, 2017·Applied Spectroscopy·Anielle Coelho RanulfiDébora Marcondes Bastos Pereira Milori
Nov 8, 2018·The Review of Scientific Instruments·D NishijimaR P Doerner

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Citations

Jan 24, 2021·Scientific Reports·Konstantinos KalerisJohn Mourjopoulos

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Methods Mentioned

BETA
PCR
PCA
chip

Software Mentioned

JMP
SpectraSuite

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