Laser microdissection-based microproteomics of the hippocampus of a rat epilepsy model reveals regional differences in protein abundances

Scientific Reports
Amanda M do CantoIscia Lopes-Cendes

Abstract

Mesial temporal lobe epilepsy (MTLE) is a chronic neurological disorder affecting almost 40% of adult patients with epilepsy. Hippocampal sclerosis (HS) is a common histopathological abnormality found in patients with MTLE. HS is characterised by extensive neuronal loss in different hippocampus sub-regions. In this study, we used laser microdissection-based microproteomics to determine the protein abundances in different regions and layers of the hippocampus dentate gyrus (DG) in an electric stimulation rodent model which displays classical HS damage similar to that found in patients with MTLE. Our results indicate that there are differences in the proteomic profiles of different layers (granule cell and molecular), as well as different regions, of the DG (ventral and dorsal). We have identified new signalling pathways and proteins present in specific layers and regions of the DG, such as PARK7, RACK1, and connexin 31/gap junction. We also found two major signalling pathways that are common to all layers and regions: inflammation and energy metabolism. Finally, our results highlight the utility of high-throughput microproteomics and spatial-limited isolation of tissues in the study of complex disorders to fully appreciate the l...Continue Reading

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Citations

Jan 26, 2021·Frontiers in Molecular Neuroscience·Amanda M do CantoIscia Lopes-Cendes
Feb 7, 2021·Proteomics·Michal AlexovičRémi Longuespée
Feb 20, 2021·Seizure : the Journal of the British Epilepsy Association·Jose Eduardo Peixoto-Santos, Ingmar Blumcke
Mar 17, 2021·Seizure : the Journal of the British Epilepsy Association·Estela M BruxelIscia Lopes-Cendes

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Methods Mentioned

BETA
GTPases
Protein Assay

Software Mentioned

ML
Adobe Photoshop
vDG
Bioconductor
MSstats R /
STRING
GL
Metacore®

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