Laser microirradiation of kinetochores in mitotic PtK2 cells: chromatid separation and micronucleus formation

Cell Biophysics
S L BrennerM W Berns

Abstract

Irradiation of the kinetochore region of PtK2 chromosomes by laser light of 532 nm was used to study the function of the kinetochore region in chromosome movement and to create an artificial micronuclei in cells. When the sister kinetochores of a chromosome were irradiated at prometaphase, the affected chromosome detached from the spindle and exhibited no further directed movements for the duration of mitosis. The chromatids of the chromosome remained attached to one another until anaphase, at which point they separated. No poleward movement of the chromatids was observed, and at telophase they passively moved to one of the daughter cells and were enclosed in a micronucleus. The daughter cell containing the micronucleus was then isolated by micromanipulation and followed through subsequent mitoses. At the next mitosis, two chromosomes, each with two chromatids, condensed in the micronucleus. These chromosomes did not attach to the spindle and showed chromatid separation, but no poleward movements at anaphase. They were again enclosed in micronuclei at telophase. The third generation mitosis was similar to the second. Occasionally, both the irradiation-produced and naturally occurring micronuclei exhibited no chromosome condensa...Continue Reading

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Citations

Mar 1, 1981·The Journal of Cell Biology·P A McNeill, M W Berns
Jan 1, 1990·International Journal of Radiation Biology·P Uma Devi, A S Sharma
Feb 1, 1991·International Journal of Radiation Biology·U Weissenborn, C Streffer
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Jan 1, 1986·Radiation and Environmental Biophysics·W U Müller, C Streffer
Feb 22, 2017·Biology·Helder MaiatoMarin Barisic
Jul 25, 2019·Molecular Biology of the Cell·Che-Hang YuDaniel Needleman
Oct 20, 2020·Frontiers in Bioengineering and Biotechnology·Alfonso Blázquez-CastroJavier Santos

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