PMID: 8603864Apr 1, 1996Paper

Laser trabeculoplasty induces stromelysin expression by trabecular juxtacanalicular cells

Investigative Ophthalmology & Visual Science
D E ParshleyT S Acott

Abstract

The mechanism by which laser trabeculoplasty reduces elevated intraocular pressure in primary open-angle glaucoma has been established. To test the hypothesis that trabecular extracellular matrix turnover is involved, stromelysin expression after laser treatment of anterior segment organ cultures was evaluated. Argon laser trabeculoplasty, using typical clinical treatment parameters, was applied to anterior segment organ cultures. Stromelysin levels and activity were then evaluated at various times by immunoblots of Western transfers and by zymography. Stromelysin mRNA levels were evaluated by dot blot and by reverse transcription, followed by polymerase chain reaction amplification. Stromelysin protein was localized by immunohistochemistry, and image analysis was used for quantitation. Stromelysin mRNA was localized by in situ hybridization. Trabecular stromelysin protein, activity, and mRNA levels were detectably elevated by 8 hours and were several-fold higher by 24 hours after treatment. Stromelysin immunostaining was elevated dramatically in the juxtacanalicular and insert regions of the meshwork, but only modestly in other regions. Stromelysin mRNA increases also were localized primarily to these regions. The juxtacanalic...Continue Reading

Citations

Jul 23, 1999·Survey of Ophthalmology·P L KaufmanM Cynader
Jun 8, 2002·Survey of Ophthalmology·Tina T L WongPeng T Khaw
Jan 18, 2006·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Esther Arranz-Marquez, Miguel A Teus
Aug 19, 2004·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fagang JiangDesheng Xu
Dec 18, 2008·Experimental Eye Research·Kate E KellerTed S Acott
Sep 8, 2007·Experimental Eye Research·Douglas H Johnson
Dec 18, 2013·Clinical & Experimental Ophthalmology·Dov B KaganCindy M L Hutnik
May 1, 2010·Lasers in Surgery and Medicine·John P M WoodRobert J Casson
May 26, 2004·Lancet·Robert N Weinreb, Peng Tee Khaw
Mar 4, 2008·Experimental Eye Research·Ted S Acott, Mary J Kelley
Jul 13, 2011·PloS One·Alberto IzzottiSergio Claudio Saccà
Mar 7, 2006·Experimental Eye Research·Xun ZhangM Elizabeth Fini
Mar 1, 2017·Clinical & Experimental Ophthalmology·Alexis Galeno MatosJayter Silva Paula

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