LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression.

Infection and Immunity
M J GambelloB H Iglewski

Abstract

The lasR gene of Pseudomonas aeruginosa is required for transcription of the genes for elastase (lasB) and LasA protease (lasA), two proteases associated with virulence. We report here that the alkaline protease gene (apr) also requires the lasR gene for transcription. Alkaline protease mRNA was absent in the lasR mutant PAO-R1 and present when an intact lasR gene was supplied in trans as determined by Northern (RNA) analysis. The lasR gene also enhances exotoxin A production. Exotoxin A activity in supernatants of PAO-R1 were 30% less than in supernatants of the parental strain, PAO-SR. Multiple copies of lasR in trans in PAO-R1 in increased toxin A activity to twice the parental levels. Analysis of PAO-R1 containing the toxA promoter fused to beta-galactosidase suggests that LasR acts at the toxA promoter or at upstream toxA mRNA sequences. beta-Galactosidase activity was approximately 40% lower in PAO-R1 than in the parental strain, PAO-SR. Furthermore, the effect of LasR on the toxA promoter is not due to the stimulation of transcription of regA, a transcriptional activator of toxA. No difference in chloramphenicol acetyltransferase (CAT) activity was noted between PAO-SR and PAO-R1 containing transcriptional regA promoter-...Continue Reading

References

Mar 1, 1979·Microbiological Reviews·B W HollowayA F Morgan
Jun 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B H Iglewski, D Kabat
Mar 1, 1990·Molecular Microbiology·D G StoreyB H Iglewski
Dec 1, 1986·Journal of Bacteriology·C C Grant, M L Vasil
Apr 1, 1985·Canadian Journal of Microbiology·T I Nicas, B H Iglewski
Jun 24, 1988·Nucleic Acids Research·M S HindahlB H Iglewski
Jan 1, 1983·Methods in Enzymology·J Messing
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Sep 1, 1980·Infection and Immunity·M K Johnson, D Boese-Marrazzo

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Citations

Oct 1, 1995·Journal of Industrial Microbiology·P J EgintonP Gilbert
Mar 12, 2002·FEMS Microbiology Letters·Barry MiddletonAndrea Hardman
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
May 31, 2003·Journal of Bacteriology·Florence JudeChristian van Delden
Mar 20, 2003·Journal of Bacteriology·Victoria E WagnerBarbara H Iglewski
Apr 10, 2004·Microbiology·Michael HogardtJürgen Heesemann
May 13, 2005·Journal of Medical Microbiology·Yoshifumi ImamuraShigeru Kohno
Jun 22, 2005·Journal of Bacteriology·Dana S WadeEverett C Pesci
Aug 10, 2005·Treatments in Respiratory Medicine·Hiroyuki Kobayashi
Apr 24, 2007·Journal of Bacteriology·Masanori ToyofukuHiroo Uchiyama
Oct 24, 2007·Marine Biotechnology·Mette Elena SkindersoeMichael Givskov
Jun 21, 2012·FEMS Microbiology Reviews·Catarina S PereiraKarina B Xavier
Aug 26, 2018·Applied Microbiology and Biotechnology·Mariam BadoJason A Rosenzweig
Feb 6, 2020·Current Microbiology·Hisham A AbbasDalia Eldamasi
Nov 10, 2001·Annual Review of Phytopathology·H CaoL G Rahme
Oct 22, 2003·Canadian Journal of Microbiology·Nancy L CartyA N Hamood
Nov 12, 2002·Journal of Bacteriology·Larry A GallagherColin Manoil
Jul 15, 2009·Biotechnology and Applied Biochemistry·Sheetal RainaTajalli Keshavarz
Sep 17, 2011·Journal of Medical Microbiology·Tim C R ConibearHua Zhu
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Devendra H DusanePattanathu K S M Rahman
Apr 14, 2012·Organic & Biomolecular Chemistry·James T HodgkinsonDavid R Spring
Feb 18, 2012·RNA Biology·Elisabeth SonnleitnerUdo Bläsi
Mar 2, 2013·Microbiology·Tsiry RasamiravakaOlivier M Vandeputte
Jul 16, 2014·Frontiers in Plant Science·Angelika Holm, Elena Vikström
Dec 10, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hui FanHaiwei Song
Nov 17, 2016·Proceedings of the National Academy of Sciences of the United States of America·Nina M Høyland-KroghsboBonnie L Bassler

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