Lasting effects of early exposure to temperature on the gonadal transcriptome at the time of sex differentiation in the European sea bass, a fish with mixed genetic and environmental sex determination

BMC Genomics
Noelia Díaz, Francesc Piferrer

Abstract

Sex in fish is plastic and in several species can be influenced by environmental factors. In sensitive species, elevated temperatures have a masculinizing effect. Previous studies on the effects of temperature on gene expression have been restricted to a few cognate genes, mostly related to testis or ovarian development, and analyzed in gonads once they had completed the process of sex differentiation. However, studies on the effect of temperature at the whole gonadal transcriptomic level are scarce in fish and, in addition, temperature effects at the time of sex differentiation at the transcriptomic level are also unknown. Here, we used the European sea bass, a gonochoristic teleost with a polygenic sex determination system influenced by temperature, and exposed larvae to elevated temperature during the period of early gonad formation. Transcriptomic analysis of the gonads was carried out about three months after the end of temperature exposure, shortly after the beginning of the process of sex differentiation. Elevated temperature doubled the number of males with respect to untreated controls. Transcriptomic analysis of early differentiating female gonads showed how heat caused: 1) an up-regulation of genes related to cholest...Continue Reading

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Citations

Jan 25, 2017·Proceedings of the National Academy of Sciences of the United States of America·Laia RibasFrancesc Piferrer
Dec 3, 2016·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Jean-François Baroiller, Helena D'Cotta
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Jul 13, 2021·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Francesc Piferrer

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Methods Mentioned

BETA
dissect
PCR
Feature Extraction
thermal treatments

Software Mentioned

Blast2GO
Feature Extraction
SDS
SPSS Statistics
DAVID
Bioconductor
limma
Agilent
RQ Manager
R

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