PMID: 6172598Nov 1, 1981Paper

Late nonstructural 100,000- and 33,000-dalton proteins of adenovirus type 2. II. Immunological and protein chemical analysis

Journal of Virology
C Gambke, W Deppert

Abstract

For an immunological analysis of the late adenovirus type 2 nonstructural 100,000-dalton (100K) and 33K proteins, we prepared antisera against sodium dodecyl sulfate-denatured, gel-purified 100K and 33K proteins. These antisera were tested for potential cross-reactivity, since according to a previous report (Axelrod, Virology 87:366--383, 1978) these two proteins exhibit extensive amino acid homologies. However, immunoprecipitations of 100K and 33K proteins, as well as a sensitive immune replica technique, did not reveal any immunological relationship between these proteins. Therefore, using fingerprint peptide analysis, we investigated the structural relationship between 100K and 33K proteins labeled with a 14C-amino acid mixture or with [14C]proline after digestion with trypsin. We detected only minor, if any, amino acid homologies, indicating that the 100K and 33K proteins are not structurally related.

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·W Deppert, G Walter
Aug 15, 1978·Analytical Biochemistry·W M Bonner, J D Stedman
Jan 1, 1978·Journal of Immunological Methods·J W Goding
Feb 1, 1979·Virology·H PerssonU Pettersson
Jun 15, 1978·Virology·N Axelrod
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J B LewisR F Gesteland
Jun 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E Lazarides, K Weber
Aug 1, 1973·Journal of Virology·C W AndersonR F Gesteland
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

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Citations

Jul 1, 2006·Journal of Virology·Philomena OstapchukPatrick Hearing

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