Late promoter selection in the baculovirus gp64 envelope fusion protein gene

Virology
D B GarrityG W Blissard

Abstract

The upstream promoter region of the Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) gp64 gene contains five copies of TAAG, the conserved sequence found at the transcriptional initiation sites of almost all baculovirus late genes. In AcMNPV-infected Sf9 cells, late transcription initiation is detected from only two upstream TAAG sites and not from three downstream TAAG sites. To examine several models for preferential TAAG site utilization, we constructed a series of recombinant AcMNPV baculoviruses that contain promoter region sequences from the gp64 gene fused to a chloramphenicol acetyl transferase reporter gene. Promoter-reporter constructs were inserted in the polyhedrin locus. To test a scanning model in which TAAG sites are sequentially selected according to their location in the region, we generated recombinant viruses in which the highly transcribed sites were inactivated by point mutations. Transcription from the mutant promoter constructs was compared qualitatively and quantitatively to transcription from the wild-type gp64 promoter. Inactivation of the upstream TAAG sites did not result in increased transcription from the downstream TAAG sites, suggesting that immediate context, rather than po...Continue Reading

References

Sep 1, 1991·Virology·M D TomalskiL K Miller
Jan 1, 1990·Annual Review of Entomology·G W Blissard, G F Rohrmann
Jan 1, 1989·The Journal of General Virology·U Weyer, R D Possee
Aug 1, 1986·The Journal of General Virology·G F Rohrmann
Mar 25, 1994·Gene·T D Morris, L K Miller
Jul 1, 1994·Journal of Virology·A L Passarelli, L K Miller
Sep 1, 1996·Trends in Genetics : TIG·C D Novina, A L Roy
Sep 1, 1996·Trends in Biochemical Sciences·R G Roeder
Nov 20, 1996·AIDS Research and Human Retroviruses·R GeleziunasW C Greene
Jan 1, 1996·Cytotechnology·G W Blissard

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Citations

Dec 10, 1999·Molecular Biology of the Cell·D H KingsleyJ Zimmerberg
Jun 29, 1999·Biological Chemistry·R M Mans, D Knebel-Mörsdorf
Feb 6, 2016·BMC Genomics·Daniel M P Ardisson-AraújoJosé L C Wolff
Mar 22, 2002·The Journal of General Virology·Margot N PearsonGeorge F Rohrmann
Feb 11, 2004·The Journal of General Virology·Serafín GutiérrezMiguel López Ferber
Oct 6, 1997·Journal of Virology·M J Chang, G W Blissard
Jun 5, 2021·Protein Expression and Purification·Carissa GroseDominic Esposito

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