Late thymectomy in Xenopus tadpoles reveals a population of T cells that persists through metamorphosis

Developmental and Comparative Immunology
L A Rollins-SmithP J Blair

Abstract

To investigate the persistence of larval T lymphocytes in the adult period, tadpoles of the South African clawed frog, Xenopus laevis, were allowed to develop to prometamorphic stages 57-58 and thymectomized (Tx). Thymectomy at this stage allows for maximal expansion of the larval T cell population but prevents emergence of the adult T cell population. Using a T cell-specific monoclonal antibody (mAb) which recognizes the XTLA-1 determinant, we examined the absolute numbers of thymic and splenic T cells expressing XTLA-1 in normal tadpoles, postmetamorphic Tx frogs, and intact age-matched adult frogs. A small, but measurable, number of larvally-derived XTLA-1+ cells persists through metamorphosis. By simultaneously staining with a mAb specific for class II major histocompatibility (MHC) antigens, we determined the phenotype of the persisting XTLA-1+ cells in the Tx frogs. Like XTLA-1+ splenocytes in intact adult controls which are predominantly class II+, most XTLA-1+ cells in Tx adults also express class II. In contrast, most XTLA-1+ cells in the tadpole are class II-. This suggests that a small population of class II+ larval T cells survives metamorphic transition to become a long-lived population in the adult. Alternatively,...Continue Reading

References

Jan 1, 1992·Developmental Immunology·L A Rollins-SmithA T Davis
Jan 1, 1990·Developmental Immunology·L Du Pasquier, M F Flajnik
May 1, 1974·European Journal of Immunology·R J Turner, M J Manning
Jan 1, 1983·Developmental and Comparative Immunology·C Kaye, R Tompkins

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Citations

Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Katsuki MukaigasaYumi Izutsu
Jan 23, 1999·Immunological Reviews·L A Rollins-Smith
Mar 3, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jacques Robert, Yuko Ohta
May 18, 2018·Cold Spring Harbor Protocols·Sara MashoofMichael F Criscitiello

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