PMID: 8971019Dec 1, 1996Paper

Late virus replication events in microglia are required for neurovirulent retrovirus-induced spongiform neurodegeneration: evidence from neural progenitor-derived chimeric mouse brains

Journal of Virology
W P LynchA H Sharpe

Abstract

CasBrE is a neurovirulent murine retrovirus which induces a spongiform myeloencephalopathy in susceptible mice. Genetic mapping studies have indicated that sequences responsible for neurovirulence reside within the env gene. To address the question of direct envelope protein neuroxicity in the central nervous system (CNS), we have generated chimeric mice expressing the CasBrE envelope protein in cells of neuroectodermal origin. Specifically, the multipotent neural progenitor cell line C17.2 was engineered to express the CasBrE env gene as either gp70/p15E (CasE) or gp70 alone (CasES). CasE expression in these cells resulted in complete (>10(5)) interference of superinfection with Friend murine leukemia virus clone FB29, whereas CasES expression resulted in a 1.8-log-unit decrease in FB29 titer. Introduction of these envelope-expressing C17.2 cells into the brains of highly susceptible IRW mice resulted in significant engraftment as integral cytoarchitecturally correct components of the CNS. Despite high-level envelope protein expression from the engrafted cells, no evidence of spongiform neurodegeneration was observed. To examine whether early virus replication events were necessary for pathogenesis, C17.2 cells expressing whol...Continue Reading

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Citations

Dec 10, 1999·Journal of Virology·S AskovićJ L Portis
Nov 12, 2003·Journal of Virology·Derek E DimcheffJohn L Portis
Feb 7, 2020·Stem Cells and Development·Cameron PerniaEvan Y Snyder
Nov 16, 2013·Experimental & Molecular Medicine·Brian T D TobeEvan Y Snyder
Nov 2, 2005·Neurotoxicity Research·Y PersidskyJ Haorah
Jun 17, 2005·Reviews in the Neurosciences·Esperanza Gomez-Lucia
May 11, 1999·Journal of Virology·C A BakerL Manuelidis
Oct 20, 1998·Seminars in Pediatric Neurology·L L BillinghurstE Y Snyder

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