Jun 1, 1977

Latent activity of liposomal creatine phosphokinase

Biulleten' eksperimental'noĭ biologii i meditsiny
E F DavidenkovaE I Shvarts


Quantitative regimen for the entrapping of creatine phosphokinase (CPK) under different experimental conditions was investigated for the preparation of liposomes with a high internal protein content. As revealed, introduction of 20% of positively-charged lipid (stearyl amine) into the lipid mixture and subsequent one-minute sonication resulted in maximal levels of latent enzyme activity. Maximal entrapment (6.1%) was yielded when 300 mM glucose solution (pH 7.0) AND 0.5 MG OF PROTEIN PER ML WERE USED. Liposome preparations when washed twice at 205 000 g with physiological saline showed minimal external enzyme activity. During storage of liposomes for 7 days under argon protection the internal protein content has not diminished, nor the external activity has increased.

  • References8
  • Citations4


Mentioned in this Paper

Macro-Creatine Kinase
Creatine Kinase Measurement
Enzyme Activity
Blood Enzyme Activity (Lab Test)

About this Paper

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Head And Neck Squamous Cell Carcinoma

Squamous cell carcinomas account for >90% of all tumors in the head and neck region. Head and neck squamous cell carcinoma incidence has increased dramatically recently with little improvement in patient outcomes. Here is the latest research on this aggressive malignancy.

Signaling in Adult Neurogenesis

Neural stem cells play a critical role in the production of neuronal cells in neurogenesis is of great importance. Of interest is the role signalling mechanisms in adult neurogenesis. Discover the latest research on signalling in adult neurogenesis.

Psychiatric Chronotherapy

Psychiatric Chronotherapy considers the circadian rhythm as a major factor for optimizing therapeutic efficacy of psychiatric interventions. Discover the latest research on Psychiatric Chronotherapy here.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.