Jun 1, 1977

Latent activity of liposomal creatine phosphokinase

Biulleten' eksperimental'noĭ biologii i meditsiny
E F DavidenkovaE I Shvarts

Abstract

Quantitative regimen for the entrapping of creatine phosphokinase (CPK) under different experimental conditions was investigated for the preparation of liposomes with a high internal protein content. As revealed, introduction of 20% of positively-charged lipid (stearyl amine) into the lipid mixture and subsequent one-minute sonication resulted in maximal levels of latent enzyme activity. Maximal entrapment (6.1%) was yielded when 300 mM glucose solution (pH 7.0) AND 0.5 MG OF PROTEIN PER ML WERE USED. Liposome preparations when washed twice at 205 000 g with physiological saline showed minimal external enzyme activity. During storage of liposomes for 7 days under argon protection the internal protein content has not diminished, nor the external activity has increased.

  • References8
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Citations

Mentioned in this Paper

Amines
Macro-Creatine Kinase
Creatine Kinase Measurement
Argon
Enzyme Activity
Blood Enzyme Activity (Lab Test)
Catalysis
Niosomes
Liposomes
Lipids

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