Latent class analysis of bulk tank milk PCR and ELISA testing for herd level diagnosis of Mycoplasma bovis

Preventive Veterinary Medicine
Per Kantsø NielsenNils Toft

Abstract

The bacterium Mycoplasma bovis causes disease in cattle of all ages. An apparent increase in the occurrence of M. bovis associated outbreaks among Danish dairy cattle herds since 2011 has prompted a need for knowledge regarding herd-level diagnostic performance. Therefore, the objective of this study was to evaluate the herd-level diagnostic performance of an indirect ELISA test by comparison to a real-time PCR test when diagnosing M. bovis in cattle herds of bulk tank milk. Bulk tank milk samples from Danish dairy herds (N=3437) were analysed with both the antibody detecting BIO K 302 M. bovis ELISA kit and the antigen detecting PathoProof Mastitis Major-3 kit. As none of these are considered a gold standard test for herd-level diagnostics we applied a series of Bayesian latent class analyses for a range of ELISA cut-off values. The negative and positive predictive values were calculated for hypothetical true national prevalences (1, 5, 10, 15 and 20%) of infected herds. We estimated that the ELISA test had a median sensitivity and specificity of 60.4 [37.5-96.2 95% Posterior Credibility Interval] and 97.3 [94.0-99.8 95% PCI] at the currently recommended cut-off (37% Optical density Coefficient). These changed to 43.5 [21.1-92...Continue Reading

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Citations

Apr 22, 2016·Ecology and Evolution·Karla Moreno-TorresRebecca Garabed
Oct 1, 2016·The Veterinary Journal·Robin A J NicholasInna Lysnyansky
May 18, 2016·PloS One·Yanliang BiZhijun Cao
May 22, 2018·Animal Health Research Reviews·Marisa L RotoloJeffrey J Zimmerman
Jan 24, 2020·BMC Genomics·Yael YairInna Lysnyansky
Sep 24, 2020·World Journal of Microbiology & Biotechnology·Feng ShiChuangfu Chen
Apr 20, 2018·Journal of Veterinary Internal Medicine·Alysia M ParkerJohn K House
Aug 13, 2020·Pathogens·Katarzyna DudekDariusz Bednarek

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