PMID: 9182700Jun 1, 1997Paper

Latent transforming growth factor-beta complex in Chinese hamster ovary cells contains the multifunctional cysteine-rich fibroblast growth factor receptor, also termed E-selectin-ligand or MG-160

The Biochemical Journal
A OlofssonC H Heldin

Abstract

Transforming growth factor-beta (TGF-beta) is secreted as latent high molecular mass complexes from producer cells. The N-terminal precursor remnant, also called latency-associated peptide (LAP), forms a non-covalently linked complex with TGF-beta and confers the latency to TGF-beta. In human platelets and certain other cell types, latent TGF-beta binding protein-1 (LTBP-1) is disulphide-linked to LAP, and forms complexes of more than 230 kDa. In addition, LTBP-2 and -3, which are structurally similar to LTBP-1, can be part of latent TGF-beta complexes. In Chinese hamster ovary (CHO) cells transfected with the TGF-beta1 cDNA, a major part of the latent TGF-beta secreted into the medium is a 100-kDa small latent complex containing TGF-beta and LAP. In addition, we found two other forms of latent TGF-beta complexes, i.e. a 220-kDa complex containing LTBP-1, and a 220-kDa complex containing a 140-kDa protein. Purification of the 140-kDa component, termed latent TGF-beta complexed protein-1 (LTCP-1), followed by amino acid sequencing and cDNA cloning from a CHO cell cDNA library, revealed that it is a hamster counterpart of a previously identified, multifunctional protein known as chicken cysteine-rich fibroblast growth factor (FGF...Continue Reading

Citations

Jan 29, 1999·Physiological Reviews·D Vestweber, J E Blanks
Jun 3, 2016·Cold Spring Harbor Perspectives in Biology·Ian B Robertson, Daniel B Rifkin
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Tao YangBrendan Lee
Jul 7, 2020·Engineering in Life Sciences·Abdalla A ElshereefDörte Solle
Mar 21, 2021·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Daniel RifkinFrancesco Ramirez
May 1, 2021·International Journal of Molecular Sciences·Kamil Karolczak, Cezary Watala

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