Lateral diffusion of inositol 1,4,5-trisphosphate receptor type 1 in Purkinje cells is regulated by calcium and actin filaments

Journal of Neurochemistry
Kazumi FukatsuKatsuhiko Mikoshiba

Abstract

Inositol 1,4,5-trisphosphate receptor type 1 (IP(3) R1) is an intracellular Ca(2+) release channel that plays crucial roles in the functions of Purkinje cells. The dynamics of IP(3) R1 on the endoplasmic reticulum membrane and the distribution of IP(3) R1 in neurons are thought to be important for the spatial regulation of Ca(2+) release. In this study, we analyzed the lateral diffusion of IP(3) R1 in Purkinje cells in cerebellar slice cultures using fluorescence recovery after photobleaching. In the dendrites of Purkinje cells, IP(3) R1 showed lateral diffusion with an effective diffusion constant of approximately 0.30 μm(2) /s, and the diffusion of IP(3) R1 was negatively regulated by actin filaments. We found that actin filaments were also involved in the regulation of IP(3) R1 diffusion in the spine of Purkinje cells. Glutamate or quisqualic acid stimulation, which activates glutamate receptors and leads to a Ca(2+) transient in Purkinje cells, decreased the diffusion of IP(3) R1 and increased the density of actin in spines. These findings indicate that the neuronal activity-dependent augmentation of actin contributes to the stabilization of IP(3) R1 in spines.

References

Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A MatusK Fujiwara
Mar 1, 1995·Journal of Neurochemistry·T Furuichi, K Mikoshiba
Jan 28, 1993·Nature·M J Berridge
Feb 25, 1998·Biochemical and Biophysical Research Communications·A MammotoY Takai
Jun 17, 1998·Molecular Biology of the Cell·B S WilsonR J Wojcikiewicz
Jun 10, 1998·Neuron·M FischerA Matus
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·T NagaiA Miyawaki
Apr 3, 2001·Nature Reviews. Neuroscience·S J Moss, T G Smart
May 2, 2001·Nature Reviews. Neuroscience·R C CarrollR C Malenka
Oct 16, 2001·The Journal of Physiology·J EilersA Konnerth
Jun 7, 2002·Nature·Aren J Borgdorff, Daniel Choquet
Jun 12, 2002·The Journal of Cell Biology·Takahiro FujiwaraAkihiro Kusumi
Apr 3, 2003·Nature Reviews. Neuroscience·Daniel Choquet, Antoine Triller
Sep 13, 2003·The EMBO Journal·Catherine TardinDaniel Choquet
Dec 6, 2003·The European Journal of Neuroscience·Akinori KurumaKatsuhiko Mikoshiba
Nov 13, 2004·The Journal of Biological Chemistry·Michelle Ferreri-JacobiaJ Kevin Foskett
Dec 8, 2004·The Journal of Biological Chemistry·Yoko TateishiKatsuhiko Mikoshiba
Feb 26, 2005·Biology of the Cell·Caroline CruttwellJean-Pierre Mauger
Nov 11, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tija C JacobStephen J Moss
Feb 21, 2006·Biochemical and Biophysical Research Communications·Kazumi FukatsuKatsuhiko Mikoshiba
Jul 18, 2006·Biophysical Journal·David Holcman, Antoine Triller
Aug 18, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Cécile CharrierAntoine Triller
Feb 13, 2007·Biophysical Journal·Marie-Virginie EhrenspergerMaxime Dahan

❮ Previous
Next ❯

Citations

May 10, 2011·The Journal of Biological Chemistry·Evangelia Pantazaka, Colin W Taylor
Jun 14, 2017·Proceedings of the National Academy of Sciences of the United States of America·Takeyuki SugawaraKatsuhiko Mikoshiba
Mar 7, 2013·Nature Communications·Guy Tran Van NhieuLaurent Combettes
Feb 13, 2019·Cold Spring Harbor Perspectives in Biology·David L Prole, Colin W Taylor
Jul 25, 2021·Nature Communications·Nagendra Babu ThillaiappanColin W Taylor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.