PMID: 3754155Apr 14, 1986Paper

Lateral diffusion of small solutes and partition of amphipaths in defect structures of lipid bilayers

Biochimica Et Biophysica Acta
H J MüllerH J Galla

Abstract

The excimer formation technique has been applied to investigate the mechanism of the lateral diffusion in the crystalline P beta' phase of dipalmitoylphosphatidylcholine vesicles. This became possible at low pyrene concentrations. From the shape of phase-transition curves, from the effect of pressure up to 150 bar and from the perturbation induced by cholesterol, we conclude that the free volume model could also be applied to the P beta' phase. However, the diffusion is thought to occur in defect structures that are considered to form fluid pathways between domains of crystalline lipid. Partition coefficients of amphipaths provide a basis for testing for the role of defects. The amphipath chlorpromazine partitions into fluid membranes with a partition coefficient, kp, of 3200, into the crystalline phase with kp = 200 but into the P beta' phase with a value of kp = 800. This again gives rise to the assumption that the P beta' phase contains fluid domains that behave like the fluid L alpha phase and make up about 10-20% of the total amount of lipid in the bilayer. Cholesterol is known to interfere especially with defect structures in the P beta' phase. It fills up the gaps, and therefore reduces the partition coefficient to almos...Continue Reading

References

Jul 31, 1979·The Journal of Membrane Biology·H J GallaE Sackmann
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Mabrey, J M Sturtevant
Feb 26, 1974·Biochimica Et Biophysica Acta·H J Galla, E Sackmann
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R Peters, K Beck

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Citations

Jan 1, 1987·European Biophysics Journal : EBJ·H J Müller, H J Galla
Jul 1, 1993·The Journal of Pharmacy and Pharmacology·A B SarmentoC R Oliveira
Jan 14, 2003·Biophysical Journal·Michael Katzer, William Stillwell
Jan 29, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Andrew D Hanlon, Bratoljub H Milosavljevic
Jul 10, 1987·Biochimica Et Biophysica Acta·J SchulzeH J Galla
Oct 6, 1988·Biochimica Et Biophysica Acta·E WajnbergS R Louro
Nov 3, 1988·Biochimica Et Biophysica Acta·K Tsuchida, I Hatta

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