Lateral dimerization of the E-cadherin extracellular domain is necessary but not sufficient for adhesive activity.

The Journal of Biological Chemistry
Masayuki Ozawa

Abstract

Cadherins are transmembrane glycoproteins involved in Ca(2+)-dependent cell-cell adhesion. Using L cells coexpressing E-cadherin constructs with different epitope tags, we examined the lateral dimerization of E-cadherin and its adhesive activity by co-immunoprecipitation and aggregation assays, respectively. Although the transmembrane domain is required for dimerization, tail-less constructs possessing the transmembrane domain of either N-cadherin or CD45 show dimerization and are active in aggregation assays. Two mutant constructs having either of two amino acid substitutions, W2A or substitutions that disrupt the recognition sequence for endoproteolytic enzymes involved in removal of the precursor segment, cannot form dimers and are inactive in aggregation. These monomeric proteins, like their wild-type dimerizing counterparts, retain their Ca(2+)-dependent resistance to trypsin digestion, suggesting that dimerization per se does not induce a large conformational change. Two other constructs, having either an amino acid substitution, D134A, or a C-terminal deletion of 70 amino acid residues, retain the ability to associate laterally but are inactive in aggregation assays. Staurosporine treatment of cells expressing the latter...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·B Geiger, O Ayalon
Oct 1, 1990·The Journal of Cell Biology·M Ozawa, R Kemler
Oct 11, 1990·Nucleic Acids Research·R A Reid, J J Hemperly
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M OzawaR Kemler
Mar 23, 1995·Nature·L ShapiroW A Hendrickson
Jun 6, 1994·FEBS Letters·M A Lemmon, D M Engelman
Oct 1, 1996·The Journal of Cell Biology·W M BrieherB M Gumbiner
Jun 5, 1997·Nature·K Simons, E Ikonen
Jun 24, 1997·Biochemistry·A W KochJ Engel
Aug 12, 1998·The Journal of Cell Biology·N A Chitaev, S M Troyanovsky
Apr 14, 1999·Nature Structural Biology·H TakedaS Hirohashi
May 4, 1999·The Journal of Cell Biology·S AonoM Takeichi
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SivasankarD Leckband
Jan 12, 2000·The Journal of Cell Biology·M A ThoresonA B Reynolds
Feb 9, 2000·The Journal of Cell Biology·W S ShanL Shapiro
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·W BaumgartnerD Drenckhahn
Jul 13, 2001·The Journal of Cell Biology·S Chappuis-FlamentB M Gumbiner

❮ Previous
Next ❯

Citations

Aug 30, 2011·Biochemistry·Nagamani Vunnam, Susan Pedigo
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Simon de BecoSylvie Coscoy
Aug 4, 2010·Proceedings of the National Academy of Sciences of the United States of America·Dietmar Schreiner, Joshua A Weiner
Nov 15, 2007·The Journal of Biological Chemistry·Yuan-Hung ChienDeborah Leckband
Jun 9, 2006·Molecular Biology of the Cell·Regina B TroyanovskySergey M Troyanovsky
Sep 1, 2007·Molecular Biology of the Cell·Regina B TroyanovskySergey M Troyanovsky
Feb 26, 2010·Molecular Biology of the Cell·Mariana V HernándezCarlos O Arregui
Aug 18, 2006·Molecular and Cellular Biology·Shinichi YonekuraChi-Hon Lee
Oct 31, 2003·Molecular and Cellular Biology·Regina B TroyanovskySergey M Troyanovsky
Jul 13, 2006·Annual Review of Biomedical Engineering·Deborah Leckband, Anil Prakasam
Jun 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·Chien Peter ChenBarry H Honig
Jul 19, 2005·Nature Reviews. Molecular Cell Biology·Barry M Gumbiner
Nov 23, 2011·The Journal of Cell Biology·Beate K StraubWerner W Franke
Dec 24, 2005·Cell Communication & Adhesion·Young J KimMargaret J Wheelock
Aug 4, 2004·Cellular and Molecular Life Sciences : CMLS·A W KochW Shan
Apr 20, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideki YamasakiYoshihiro Yoneda
Apr 9, 2004·The EMBO Journal·Daniel HäussingerStephan Grzesiek
Jul 27, 2010·Biophysical Journal·Quanming ShiDeborah Leckband
Apr 12, 2005·European Journal of Cell Biology·Sergey Troyanovsky
Aug 17, 2011·Biochimica Et Biophysica Acta·Nagamani VunnamSusan Pedigo
Mar 10, 2010·Cell Adhesion & Migration·Florian Cymer, Dirk Schneider
Nov 5, 2014·Biochemistry·Jared M JunglesSusan Pedigo
Feb 9, 2017·Scientific Reports·Severine A DegrelleGuillaume Pidoux
Jan 1, 2011·Genes·Francesca Soncin, Christopher M Ward
Jan 13, 2006·Journal of Neurobiology·Gallen B Triana-Baltzer, Martina Blank
Jun 18, 2017·European Biophysics Journal : EBJ·Alexandra Schumann-GillettMegan L O'Mara
Feb 27, 2003·The Journal of Biological Chemistry·James K WahlMargaret J Wheelock
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kiyono ShiraishiTsutomu Takeuchi
Jul 16, 2015·The Journal of Biological Chemistry·Nitesh ShashikanthDeborah E Leckband
Dec 24, 2005·The Journal of Pharmacology and Experimental Therapeutics·Naoki KobayashiTeruna J Siahaan
Mar 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Connor J ThompsonDaniel K Schwartz
Dec 24, 2005·Journal of Cell Science·Porntula PanorchanDenis Wirtz
Dec 7, 2007·Journal of Cell Science·Yayoi Miyashita, Masayuki Ozawa
Jan 27, 2005·Journal of Cell Science·Oliver J HarrisonPeter J Kilshaw

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.