PMID: 8946213Oct 1, 1996Paper

Lateral dynamics of major histocompatibility complex class II molecules bound with agonist peptide or altered peptide ligands

Immunology Letters
Y QiuB G Barisas

Abstract

We examined the lateral diffusion of I-Ad on A20 cells following the binding of ovalbumin-derived peptides. The peptides were OVA323-339 and OVA325-335 and a related peptide OVA325-335s substituted H331Q. Only OVA323-339 and OVA325-335 were effectively presented by A20 cells to DO-11.10/S4.4 T cells as assessed by IL-2 production. Fluorescence photobleaching recovery (FPR) measurements showed anti-I-Ad to have a lateral diffusion coefficient on untreated A20 cells of 1.8 +/- 1.0 x 10(-10) cm2 s-1 at 25 degrees C with fluorescence recovery after photobleaching greater than 50%. After 24 h incubation of A20 cells with OVA323-339 or OVA325-335, a subpopulation of A20 cells appeared that were approximately half the size of untreated A20 cells. Culture of A20 with OVA325-355s did not stimulate DO-11.10 cells or induce a size change in A20 cells. Class II molecules were laterally immobile on these small cells with fluorescence recoveries after photobleaching of less than 20%. The relative number of small cells in the A20 cell population was correlated with the immunogenicity of the peptides. These results suggest that immobilization of surface I-Ad may be an important event in antigen presentation.

References

Jul 14, 1977·Nature·S Gillis, K A Smith
Jan 1, 1978·Journal of Immunological Methods·J W Goding
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T H WattsH M McConnell
Nov 1, 1989·Scientific American·H M GreyS Buus
Aug 1, 1987·Immunological Reviews·S BuusH M Grey
Aug 1, 1983·The Journal of Experimental Medicine·R ShimonkevitzH Grey
Jun 28, 2011·Blood·Tobias A FuchsDenisa D Wagner

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Citations

Dec 11, 1999·Molecular Immunology·B G BarisasD A Roess
Feb 13, 2001·Biophysical Journal·C WofsyB Goldstein
Sep 1, 2000·International Immunology·H M MunnellyB G Barisas

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