Lateral flow assay with pressure meter readout for rapid point-of-care detection of disease-associated protein

Lab on a Chip
Bingqian LinChaoyong James Yang

Abstract

Paper-based assays such as lateral flow assays are good candidates for portable diagnostics owing to their user-friendly format and low cost. In terms of analytical detection, lateral flow assays usually require dedicated instruments to obtain quantitative results. Here we demonstrate a lateral flow assay with handheld pressure meter readout for the rapid detection of disease-related protein with high sensitivity and selectivity. Based on the pressure change produced by the catalytic reaction of Pt nanoparticles related to the concentration of the target, a quantitative reaction platform was established. During the lateral flow assay, the Pt nanoparticles are aggregated in the test line to form a gray band by biomolecular recognition and finally convert the recognition signal into highly sensitive pressure readout for quantitative analysis. Without sophisticated instrumentation and complicated operations, the whole detection process can be completed within 20 minutes. The limit of detection for myoglobin (2.9 ng mL-1 in diluted serum samples) meets the requirements of clinical monitoring. With the advantages of low cost, ease of operation, high sensitivity and selectivity, the method represents a versatile platform for point-of...Continue Reading

References

Jan 9, 2009·Analytical and Bioanalytical Chemistry·Axel Warsinke
Mar 15, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V V ShumiantsevaA I Archakov
Dec 20, 2012·Nature Communications·Yujun SongLidong Qin
Nov 2, 2013·Biosensors & Bioelectronics·Hyou-Arm JoungMin-Gon Kim
Dec 4, 2014·Analytica Chimica Acta·Jong-Min ParkJae-Chul Pyun
Jul 2, 2016·Essays in Biochemistry·Katarzyna M Koczula, Andrea Gallotta
Jun 1, 2011·Trends in Analytical Chemistry : TRAC·Peter B LuppaHarald Schlebusch

❮ Previous
Next ❯

Citations

Aug 1, 2018·Lab on a Chip·Peter ThurgoodKhashayar Khoshmanesh
Sep 15, 2018·International Journal of Molecular Sciences·Joshua M CampbellAdam T Melvin
Jun 30, 2019·Lab on a Chip·Joshua D BishopBernhard H Weigl
Sep 14, 2018·Biosensors & Bioelectronics·Lixu WangHuanghao Yang
Nov 10, 2018·Analytical Chemistry·Yanling SongChaoyong Yang
Nov 18, 2021·Analytical Methods : Advancing Methods and Applications·Tong ShuMichael J Serpe

❮ Previous
Next ❯

Methods Mentioned

BETA
chips
ELISA
chip

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.