Lateral inhibition: Two modes of non-autonomous negative autoregulation by neuralized.

PLoS Genetics
Steven W Miller, J W Posakony

Abstract

Developmental patterning involves the progressive subdivision of tissue into different cell types by invoking different genetic programs. In particular, cell-cell signaling is a universally deployed means of specifying distinct cell fates in adjacent cells. For this mechanism to be effective, it is essential that an asymmetry be established in the signaling and responding capacities of the participating cells. Here we focus on the regulatory mechanisms underlying the role of the neuralized gene and its protein product in establishing and maintaining asymmetry of signaling through the Notch pathway. The context is the classical process of "lateral inhibition" within Drosophila proneural clusters, which is responsible for distinguishing the sensory organ precursor (SOP) and non-SOP fates among adjacent cells. We find that neur is directly regulated in proneural clusters by both proneural transcriptional activators and Enhancer of split basic helix-loop-helix repressors (bHLH-Rs), via two separate cis-regulatory modules within the neur locus. We show that this bHLH-R regulation is required to prevent the early, pre-SOP expression of neur from being maintained in a subset of non-SOPs following SOP specification. Lastly, we demonstr...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C Delidakis, S Artavanis-Tsakonas
Oct 22, 1982·Science·G M Rubin, A C Spradling
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·M KunischJ A Campos-Ortega
Jul 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J B Skeath, S B Carroll
Nov 7, 2001·Current Biology : CB·E YehG L Boulianne
Dec 11, 2003·Genes & Development·Hamed Jafar-NejadHugo J Bellen
Aug 7, 2004·Science·Dave KosmanEthan Bier
Oct 16, 2004·Molecular and Cellular Biology·Lynn M PowellAndrew P Jarman
Feb 26, 2005·Nucleic Acids Research·Søren WarmingNeal G Copeland
Mar 2, 2005·Developmental Cell·Nick Reeves, James W Posakony
May 17, 2005·Developmental Biology·Mark RebeizJames W Posakony
Aug 12, 2005·Development·Chrysoula Pitsouli, Christos Delidakis
Feb 7, 2006·Developmental Cell·Allison J Bardin, François Schweisguth
Feb 7, 2006·Developmental Cell·Stefano De RenzisEric Wieschaus
Aug 22, 2006·Nature Reviews. Molecular Cell Biology·Sarah J Bray
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
May 19, 2007·Nature Reviews. Genetics·Uri Alon
Nov 15, 2007·PloS One·Savita AyyarPat Simpson
Aug 30, 2008·Current Opinion in Genetics & Development·Lynn M Powell, Andrew P Jarman
Jul 8, 2010·Science Signaling·Omer BaradNaama Barkai
Jul 31, 2010·Proceedings of the National Academy of Sciences of the United States of America·Hervé RouaultFrançois Schweisguth
Aug 28, 2010·Current Biology : CB·Michael W PerryMichael Levine
Feb 1, 2011·Current Biology : CB·Akiko KotoMasayuki Miura
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Steven W MillerJames W Posakony

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BETA
electrophoretic mobility shift assay

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MARCM
Leica confocal
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