Lateral mobility of plasma membrane lipids in Xenopus eggs: regional differences related to animal/vegetal polarity become extreme upon fertilization

Developmental Biology
W J DictusJ G Bluemink

Abstract

Regional differences in the lateral mobility properties of plasma membrane lipids have been studied in unfertilized and fertilized Xenopus eggs by fluorescence photobleaching recovery (FPR) measurements. Out of a variety of commonly used lipid probes only the aminofluorescein-labeled fatty acids HEDAF (5-(N-hexadecanoyl)-aminofluorescein) and TEDAF (5-(N-tetradecanoyl)-aminofluorescein) appear to partition into the plasma membrane. Under all experimental conditions used these molecules show partial recovery upon photobleaching indicating the existence of lipidic microdomains. In the unfertilized egg the mobile fraction of plasma membrane lipids (approximately 50%) has a fivefold smaller lateral diffusion coefficient (D = 1.5 X 10(-8) cm2/sec) in the animal than in the vegetal plasma membrane (D = 7.6 X 10(-8) cm2/sec). This demonstrates the presence of an animal/vegetal polarity within the Xenopus egg plasma membrane. Upon fertilization this polarity is strongly (greater than 100X) enhanced leading to the formation of two distinct macrodomains within the plasma membrane. At the animal side of the egg lipids are completely immobilized on the time scale of FPR measurements (D less than 10(-10) cm2/sec), whereas at the vegetal sid...Continue Reading

References

Apr 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M D Maser, J J Trimble
Sep 29, 1978·Biochimica Et Biophysica Acta·H Sandermann
Feb 1, 1978·Developmental Biology·J G Bluemink, L G Tertoolen
Nov 1, 1976·Biophysical Journal·D E KoppelW W Webb
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·J G Bluemink
Feb 18, 1972·Science·S J Singer, G L Nicolson
Jan 1, 1974·Developmental Biology·R D GreyJ L Hedrick
Jan 1, 1968·Gerontologia Clinica·R Davidson
Jan 1, 1982·Developmental Biology·D G Capco, W R Jeffrey
Feb 1, 1982·The Journal of Experimental Zoology·D G Capco
Nov 30, 1982·Biochimica Et Biophysica Acta·A I Magee, M J Schlesinger
Sep 1, 1981·Developmental Biology·R Peters, H P Richter
Dec 23, 1980·Biochemistry·R D Klausner, D E Wolf
Mar 1, 1975·Wilhelm Roux's Archives of Developmental Biology·John G Bluemink, Olga A Hoperskaya

❮ Previous
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Citations

Apr 1, 1991·Journal of Electron Microscopy Technique·J Stewart-SavageR P Elinson
Sep 6, 1994·Chemistry and Physics of Lipids·J F TocanneM Welby
Jul 1, 1984·Developmental Biology·P A TetterooS W de Laat
Jul 1, 1985·Developmental Biology·J E SpeksnijderS W de Laat
Oct 1, 1985·Developmental Biology·D Kline, R Nuccitelli
Nov 1, 1984·Cell Differentiation·J P Thiery
Jun 1, 1987·Cell Differentiation·C Sardet, P Chang
Sep 1, 1987·Cell Differentiation·G BernardiniR Stipani
Jan 1, 1994·Progress in Lipid Research·J F TocanneA Lopez
Jan 15, 1987·Molecular and Biochemical Parasitology·M W KennedyP B Garland
Jan 1, 1984·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·G A Ubbels, T G Brom
Apr 1, 1992·Comparative Biochemistry and Physiology. Comparative Physiology·A De LoofJ V vanden Broeck
Nov 1, 1989·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M Charbonneau, N Grandin
Feb 1, 1997·Parasitology Today·M C Roberts, J Modha
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A G WattsR Levenson
Sep 1, 1986·The Journal of Cell Biology·M FoleyI Moore
May 1, 1994·The Journal of Cell Biology·J L ThomasW W Webb
May 1, 1994·Zygote : the Biology of Gametes and Early Embryos·A D Roeder, D L Gard
Sep 7, 1990·Journal of Theoretical Biology·S Svetina, B Zeks
Jan 1, 1995·Molecular Membrane Biology·D E Wolf
Jan 1, 1987·CRC Critical Reviews in Biochemistry·N Dascal
Nov 26, 1998·Biology of Reproduction·C A WolfeR Jones
Oct 1, 1989·Gamete Research·G BernardiniP Belgiojoso
Nov 1, 1985·The American Journal of Anatomy·J K KoehlerD Smith
Oct 23, 1989·FEBS Letters·J F TocanneJ F Tournier
Apr 9, 1987·Biochimica Et Biophysica Acta·S N TreistmanD E Wolf
Feb 1, 1986·Experimental Cell Research·R Talevi, B Dale

❮ Previous
Next ❯

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