Lateral organization of liquid-crystalline cholesterol-dimyristoylphosphatidylcholine bilayers. Evidence for domains with hexagonal and centered rectangular cholesterol superlattices

Biochemistry
Jorma A VirtanenPentti Somerharju

Abstract

The lateral organization of fluid cholesterol-dimyristoylphosphatidylcholine (DMPC) bilayers was studied by measuring the response of fluorescent membrane probes, dipyrenylphosphatidylcholines (diPyrxPCs) or merocyanine 540, to the variation of cholesterol concentration. Parallel absorbance and light-scattering measurements were also carried out. The excimer-to-monomer ratio of diPyrxPCs displayed abrupt deviations at particular cholesterol mole fractions (CMFs). The most notable of these occurred at CMFs of 0.15, 0.33, and 0.67. Deviations were also frequently observed at CMFs of 0.12, 0.20, 0.25, and 0.40. Merocyanine 540 reproducibly reported deviations at CMFs of 0.15 and 0.33 and frequently reported values close to 0.12, 0.20, and 0.25. In absorbance (turbidity) and light scattering versus CMF plots, well-defined kinks were observed at CMFs of 0.16, 0.33, 0.52, and 0.67. The occurrence of kinks or other deviations at those particular CMFs is most readily explained in terms of a superlattice model previously developed to explain the lateral distribution of pyrenylphospholipids in bilayers [Somerharju, et al. (1985) Biochemistry 24, 2773-2781; Virtanen, J. A., et al. (1988) J. Mol. Electron. 4, 233-236]. This model is based ...Continue Reading

Citations

Jul 3, 2002·Chemistry and Physics of Lipids·Parkson Lee-Gau Chong, István P Sugár
Nov 6, 2001·Progress in Lipid Research·Henna Ohvo-RekiläJ Peter Slotte
May 5, 2001·International Journal of Pharmaceutics·J Y FangY Y Wang
Oct 18, 2001·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·S MazèresA Lopez
Sep 9, 1999·Biochimica Et Biophysica Acta·P SomerharjuK H Cheng
Dec 27, 2011·Journal of the American Chemical Society·István P Sugár, Parkson L-G Chong
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·A Radhakrishnan, H M McConnell
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J A VirtanenP Somerharju
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Md Rejwan AliJuyang Huang
Feb 27, 2014·Drug Development and Industrial Pharmacy·M J Alvarez-FigueroaM A Soto-Arriaza
Oct 15, 2008·Drug Development and Industrial Pharmacy·M Adolfina RuizVisitacion Gallardo
Mar 16, 2011·Journal of Pharmaceutical Sciences·Raju JukantiPrabhakar R Veerareddy
Sep 29, 2009·Colloids and Surfaces. B, Biointerfaces·Lorena TavanoNevio Picci
Mar 27, 2009·Biophysical Chemistry·Cezary WatalaDzmitry Shcharbin
May 27, 2008·Colloids and Surfaces. B, Biointerfaces·Nobutake TamaiShoji Kaneshina
Nov 15, 2008·Biochimica Et Biophysica Acta·Pentti SomerharjuMartin Hermansson
Nov 18, 2008·Biochimica Et Biophysica Acta·Parkson Lee-Gau ChongBerenice Venegas
Mar 12, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Ibrahim A AlsarraG M Mahrous
Feb 8, 2006·Biophysical Journal·Benjamin L Stottrup, Sarah L Keller
Aug 12, 2008·Biophysical Journal·Berenice VenegasParkson Lee-Gau Chong
Aug 13, 2008·Analytical Biochemistry·Michelle Olsher, Parkson Lee-Gau Chong
Jun 12, 2013·Biophysical Journal·István P SugárParkson L-G Chong
May 1, 1997·Archives of Biochemistry and Biophysics·D DumasJ F Stoltz
May 2, 1998·Biochimica Et Biophysica Acta·T X Xiang, B D Anderson
Mar 22, 2003·Biochimica Et Biophysica Acta·Harden M McConnell, Arun Radhakrishnan
Mar 22, 2003·Biochimica Et Biophysica Acta·John R Silvius
Mar 17, 1999·Biochimica Et Biophysica Acta·J HuangG W Feigenson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.