PMID: 20928940Oct 12, 2010

Laterally attached kinetochores recruit the checkpoint protein Bub1, but satisfy the spindle checkpoint

Cell Cycle
Michelle M ShimogawaTrisha N Davis

Abstract

Kinetochore attachment to the ends of dynamic microtubules is a conserved feature of mitotic spindle organization that is thought to be critical for proper chromosome segregation. Although kinetochores have been described to transition from lateral to end-on attachments, the phase of lateral attachment has been difficult to study in yeast due to its transient nature. We have previously described a kinetochore mutant, DAM1-765, which exhibits lateral attachments and misregulation of microtubule length. Here we show that the misregulation of microtubule length in DAM1-765 cells occurs despite localization of microtubule associated proteins Bik1, Stu2, Cin8, and Kip3 to microtubules. DAM1-765 kinetochores recruit the spindle checkpoint protein Bub1, however Bub1 localization to DAM1-765 kinetochores is not sufficient to cause a cell cycle arrest. Interestingly, the DAM1-765 mutation rescues the temperature sensitivity of a biorientation-deficient ipl1-321 mutant, and DAM1-765 chromosome loss rates are similar to wild-type cells. The spindle checkpoint in DAM1-765 cells responds properly to unattached kinetochores created by nocodazole treatment and loss of tension caused by a cohesin mutant. Progression of DAM1-765 cells through m...Continue Reading

References

May 24, 2005·Experimental Cell Research·Loleta HarrisRakesh Goorha
Dec 4, 2001·Biology of the Cell·James L MallerBrian J Tunquist
May 4, 2002·Molecular and Cellular Endocrinology·James L MallerBrian J Tunquist
May 10, 2000·Current Opinion in Cell Biology·A L Pidoux, R C Allshire
Nov 8, 2001·Current Opinion in Cell Biology·B Lee, A Amon
Jun 18, 2002·Current Opinion in Cell Biology·Tomoyuki U Tanaka
Dec 22, 1999·Current Opinion in Cell Biology·W Zachariae
Feb 13, 2001·Current Opinion in Genetics & Development·K Wassmann, R Benezra
Mar 16, 2001·Current Opinion in Genetics & Development·K F Sullivan
Feb 19, 2000·Current Opinion in Genetics & Development·D J Burke
Feb 19, 2000·Current Opinion in Genetics & Development·L Cerutti, V Simanis
May 24, 2003·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J P A BaakE A M Janssen
Sep 22, 2001·Current Biology : CB·B M Stern, A W Murray
Mar 31, 2000·Trends in Cell Biology·R D Gardner, D J Burke
Apr 8, 2000·Trends in Cell Biology·K H Choo
Dec 20, 2000·Trends in Plant Science·H G YuR K Dawe
Apr 12, 2005·Reproductive Biomedicine Online·Jan Tesarik
Mar 11, 2003·Reproductive Biomedicine Online·Jan Tesarik, Carmen Mendoza
Nov 11, 2006·BMC Bioinformatics·Anna LovricsBéla Novák
Jun 27, 2014·Chromosoma·Francesca Malvezzi, Stefan Westermann
May 9, 2012·Experimental Cell Research·Neil T Umbreit, Trisha N Davis
May 30, 2015·Molecular Biology of the Cell·Nathaniel I KrefmanGeorjana Barnes
Oct 11, 2014·Nature Reviews. Molecular Cell Biology·Nitobe London, Sue Biggins
May 26, 2017·The Journal of Cell Biology·Jonathan Kuhn, Sophie Dumont
Dec 15, 2017·Molecular Biology of the Cell·Régis E MeyerDean S Dawson
Apr 11, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sasikumar RajooKarsten Weis

Citations

Jun 12, 1998·The Journal of Cell Biology·J C WatersEdward D Salmon
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D A SkoufiasR L Margolis
Sep 22, 2001·Current Biology : CB·B M Stern, A W Murray
Jan 5, 2002·The Journal of Cell Biology·H LinDavid Pellman
Dec 11, 2002·The Journal of Cell Biology·Tatiana IoukRichard W Wozniak
Oct 1, 2003·Molecular Biology of the Cell·Chad G PearsonKerry Bloom
Feb 10, 2004·Nature Cell Biology·Michael A LampsonTarun M Kapoor
Feb 11, 2004·The Journal of Cell Biology·Emily S GillettPeter K Sorger
Nov 9, 2004·Current Biology : CB·Chad G PearsonKerry Bloom
Apr 23, 2005·Nature·Kozo TanakaTomoyuki U Tanaka
May 6, 2005·Molecular Biology of the Cell·Eric G MullerTrisha N Davis
Jun 4, 2005·The EMBO Journal·Patrick Meraldi, Peter K Sorger
Mar 15, 2006·The Journal of Cell Biology·Jessica D Tytell, Peter K Sorger
Mar 31, 2006·Molecular Biology of the Cell·Michael J WolyniakTim C Huffaker
Aug 8, 2006·Current Biology : CB·Michelle M ShimogawaTrisha N Davis
May 18, 2007·Molecular Biology of the Cell·Lina MaVivien Measday
Dec 6, 2008·Molecular Biology of the Cell·Michelle M ShimogawaTrisha N Davis
Jan 14, 2009·The Journal of Cell Biology·Robert J ScottRichard W Wozniak
Jul 7, 2010·Cell Cycle·Megan M WargackiTrisha N Davis
Dec 1, 1949·Journal of Genetics·D E LEA, C A COULSON

Related Concepts

Saccharomyces cerevisiae Proteins
Establishment and Maintenance of Localization
Antineoplastic Agents
Microtubule-Associated Proteins
Microtubule-Associated Protein 3
Cell Cycle Proteins
BCAS2 gene
Cohesins
BCAS2 wt Allele
Intracellular Signaling Proteins

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.