Latex agglutination assays for detection of non-O157 Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145

Journal of Food Protection
Marjorie B MedinaAndchitrita Debroy

Abstract

Latex agglutination assays utilizing polyclonal antibodies were developed for the top six non-O157 Shiga toxin-producing Escherichia coli (STEC) serogroups. Rabbit antisera were affinity purified through protein A/G columns, and the isolated immunoglobulins (IgGs) were covalently immobilized onto polystyrene latex particles. The resulting latex-IgG complex had a protein (IgG) load of 0.20 to 0.28 mg/ml in a 1% latex suspension. Optimum conditions for the agglutination assay consisted of utilizing 20 μm l of latex-IgG reagent containing 2.0 to 2.8 μm g IgG in a 0.5% latex suspension. Agglutination or flocculation was observed almost instantly after mixing the colonies with the latex-IgG, indicating STEC strains. More than 100 target and nontarget strains were tested in more than 3,000 test replicates. All target organisms produced positive results, but three antisera (anti-O26, anti-O103, and anti-O145) cross-reacted with some other STECs. The anti-O103 and anti-O145 latex reagents cross-reacted with O26 strains, and the anti-O26 cross-reacted with O103 strains. The latex-IgG reagents are stable for at least 1 year and are easy to prepare. These agglutination assays can be used for identification of presumptive non-O157 STEC col...Continue Reading

References

Apr 18, 2001·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y H HuangT C Chang
Sep 6, 2003·Journal of Clinical Microbiology·H-C SlotvedH B Konradsen
Dec 31, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John T BrooksCindy R Friedman
Jun 9, 2004·Journal of Clinical Microbiology·H-C SlotvedF Espersen
Sep 20, 2005·The Journal of Infectious Diseases·John T BrooksNancy A Strockbine
Aug 31, 2006·The Journal of Infectious Diseases·Mark E WickhamB Brett Finlay
Nov 7, 2006·Journal of Animal Science·C L Gyles
Nov 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kristine E JohnsonCynthia L Sears
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Jul 30, 2011·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons

❮ Previous
Next ❯

Citations

Feb 6, 2014·International Journal of Food Microbiology·M TrevisaniS Bonardi
Jun 13, 2013·Foodborne Pathogens and Disease·Fei WangBeilei Ge
May 7, 2013·Sensors·Andrew GehringJoseph Uknalis
Apr 25, 2017·Journal of Immunological Methods·Xiaohua HeDaniela Mavrici

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.