Apr 22, 1989

Latex agglutination test for diagnosing pneumococcal pneumonia in children in developing countries

BMJ : British Medical Journal
K P O'NeillB M Greenwood

Abstract

To prepare and assess the sensitivity and specificity of a latex agglutination test specific for the serotype of antigen in diagnosing pneumococcal pneumonia in Gambian children. Comparison of agglutination test specific for serotype with culture of blood and lung aspirates, countercurrent immunoelectrophoresis, and commercial latex agglutination tests in diagnosing pneumococcal pneumonia. Cross reaction studies and investigation of 102 control children to determine specificity of agglutination test specific for serotype. General medical ward of Medical Research Council laboratories, The Gambia. 101 Gambian children aged between 2 months and 10 years admitted with severe pneumonia. Serum samples were boiled and treated with edetic acid, and urine samples were boiled and concentrated 25 times before testing. A latex agglutination test specific for the serotype of pneumococcal antigen that is sensitive and highly specific for detecting pneumococcus in the urine of patients with pneumococcal pneumonia. Concentrated urine samples from 16 of the 21 children (76%) with pneumococcal pneumonia established by results of culture of blood or lung aspirates gave a positive result with the agglutination test specific for serotype, whereas o...Continue Reading

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Citations

Mentioned in this Paper

Urine
Lung
Cross Reactions
Aspirate Substance
Antigens, Bacterial
Antigens
Lung Diseases
Countercurrent Electrophoresis Measurement
Polyvalent pneumococcal vaccine
Edetic Acid

About this Paper

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