PMID: 11607069Mar 1, 1990Paper

Laticifer-specific gene expression in Hevea brasiliensis (rubber tree)

Proceedings of the National Academy of Sciences of the United States of America
A KushN H Chua

Abstract

Natural rubber, cis-1,4-polyisoprene, is obtained from a colloidal fluid called latex, which represents the cytoplasmic content of the laticifers of the rubber tree (Hevea brasiliensis). We have developed a method of extracting translatable mRNA from freshly tapped latex. Analysis of in vitro translation products of latex mRNA showed that the encoded polypeptides are very different from those of leaf mRNA and these differences are visible in the protein profiles of latex and leaf as well. Northern blot analysis demonstrated that laticifer RNA is 20- to 100-fold enriched in transcripts encoding enzymes involved in rubber biosynthesis. Plant defense genes encoding chitinases, pathogenesis-related protein, phenylalanine ammonia-lyase, chalcone synthase, chalcone isomerase, cinnamyl alcohol dehydrogenase, and 5-enolpyruvylshikimate-3-phosphate synthase show a 10- to 50-fold higher expression in laticifers than in leaves, indicating the probable response of rubber trees to tapping and ethylene treatment. Photosynthetic genes encoding ribulose-bisphosphate carboxylase small subunit and chlorophyll a/b-binding protein are not expressed at a detectable level in laticifers. In contrast, genes encoding two hydrolytic enzymes, cellulase a...Continue Reading

References

Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K E BroglieR M Broglie
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M H WalterC J Lamb
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K EdwardsC J Lamb
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D DellapennaA B Bennett
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J R Ecker, R W Davis

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Citations

Oct 1, 1995·Plant Molecular Biology·S SivasubramaniamN H Chua
Oct 1, 1995·Plant Molecular Biology·M L Chye, K Y Cheung
Feb 3, 2009·Journal of Chemical Ecology·Amit SethiRussell T Nagata
Aug 4, 2011·Plant Molecular Biology·Zhihui XiaXi Huang
Aug 10, 2002·Phytochemistry·Dhirayos WititsuwannakulRapepun Wititsuwannakul
Jun 25, 2002·Methods : a Companion to Methods in Enzymology·H Y YeangE Sunderasan
Feb 26, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·V P KurupJ N Fink
Dec 14, 2011·Journal of Experimental Botany·Keng-See ChowKiew-Lian Wan
Jan 18, 2013·BMC Genomics·Cuifang DuanPascal Montoro
Aug 8, 2008·Bioscience, Biotechnology, and Biochemistry·Tomoki SandoAkio Kobayashi
Feb 4, 2014·BMC Research Notes·Keng-See ChowZainorlina Mohd-Zainuddin
Jul 13, 2012·Bioscience, Biotechnology, and Biochemistry·Liu-Hong DengHui-Cai Zeng
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sung-Jin KimNorman G Lewis
May 11, 2007·Journal of Biochemical and Biophysical Methods·Chaorong TangYuekun Wang
Jul 15, 2015·Journal of Experimental Botany·Edouard Boex-FontvieilleChristiane Reinbothe
Nov 1, 1995·The Journal of Allergy and Clinical Immunology·T YagamiM Shono
Jul 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Yuichi AokiToru Nakayama
Dec 17, 2009·Plant Signaling & Behavior·Jiahong Zhu, Zhili Zhang
Nov 8, 2017·International Journal of Molecular Sciences·Xuan DongDe-Gang Zhao
Jul 2, 1998·Clinical and Experimental Immunology·D A KostyalD H Beezhold

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