Latrophilin mediates insecticides susceptibility and fecundity through two carboxylesterases, esterase4 and esterase6, in Tribolium castaneum

Bulletin of Entomological Research
Luting WeiBin Li

Abstract

Latrophilin (LPH) is known as an adhesion G-protein-coupled receptor which involved in multiple physiological processes in organisms. Previous studies showed that lph not only involved the susceptibility to anticholinesterase insecticides but also affected fecundity in Tribolium castaneum. However, its regulatory mechanisms in these biological processes are still not clear. Here, we identified two potential downstream carboxylesterase (cce) genes of Tclph, esterase4 and esterase6, and further characterized their interactions with Tclph. After treatment of T. castaneum larvae with carbofuran or dichlorvos insecticides, the transcript levels of Tcest4 and Tcest6 were significantly induced from 12 to 72 h. RNAi against Tcest4 or Tcest6 led to the higher mortality compared with the controls after the insecticides treatment, suggesting that these two genes play a vital role in detoxification of insecticides in T. castaneum. Furthermore, with insecticides exposure to Tclph knockdown beetles, the expression of Tcest4 was upregulated but Tcest6 was downregulated, indicating that beetles existed a compensatory response against the insecticides. Additionally, RNAi of Tcest6 resulted in 43% reductions in female egg laying and completely i...Continue Reading

References

May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D G Gilbert, R C Richmond
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·R D NewcombJ G Oakeshott
Aug 22, 1997·The Journal of Biological Chemistry·V G LelianovaY A Ushkaryov
Aug 19, 1999·Insect Biochemistry and Molecular Biology·C ClaudianosJ G Oakeshott
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 5, 2003·The Biochemical Journal·Christopher J MeeDavid R Bell
Nov 13, 2004·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Mao-Liang Chen, Chia-Hsiang Chen
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yuko Ishida, Walter S Leal
Oct 4, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sally E DoyleRaymond B Runyan
May 5, 2009·Trends in Neurosciences·Christoph KellendonkEric R Kandel
Jun 19, 2009·Journal of Molecular Evolution·Charles RobinJohn G Oakeshott
Sep 1, 2009·Insect Biochemistry and Molecular Biology·Khurshida BegumYoonseong Park
Jul 22, 2010·European Journal of Human Genetics : EJHG·Maria C BonagliaOrsetta Zuffardi
Jan 1, 2010·Advances in Experimental Medicine and Biology·Tobias Langenhan, Andreas P Russ
Jan 1, 2010·Advances in Experimental Medicine and Biology·John-Paul Silva, Yuri A Ushkaryov
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 26, 2013·The Journal of Biological Chemistry·Antony A BoucardThomas C Südhof
Mar 22, 2014·PLoS Neglected Tropical Diseases·Rodolphe PoupardinHilary Ranson
Nov 28, 2014·Biomolecular Concepts·Diana G Meza-Aguilar, Antony A Boucard
May 20, 2015·Pesticide Biochemistry and Physiology·Young Ho KimKun Yan Zhu
Apr 28, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Xiaowen SongBin Li
Sep 28, 2017·Journal of Genetics·Kavita Krishnamoorti, Arvind Kumar Singh
Oct 21, 2017·Genesis : the Journal of Genetics and Development·Shanshan GaoBin Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.