Layer-by-layer assembled gold nanoparticles/lower-generation (Gn≤3) polyamidoamine dendrimers-grafted reduced graphene oxide nanohybrids with 3D fractal architecture for fast, ultra-trace, and label-free electrochemical gene nanobiosensors

Biosensors & Bioelectronics
Kumarasamy JayakumarYangping Wen

Abstract

Three layer-by-layer (LBL) assembled gold nanoparticles (AuNPs)/lower-generation (Gn≤3) polyamidoamine dendrimer (PD) with reduced graphene oxide (rGO) as the core/mercaptopropinoic acid (MPA)/Au were successfully fabricated and employed as electrochemical gene nanobiosensing platforms with three-dimensional (3D) fractal nanoarchitecture for fast, ultra-trace determination of label-free DNA hybridization. Three Gn≤3PD were initially grafted to graphite oxide (GO) via the covalent functionalization between amino terminals of PD and carboxyl terminals of GO where a concomitant reduction of GO, which were covalently linked onto MPA that was self-assembled onto Au substrate, and finally AuNPs were encapsulated onto GG1PD by strong physicochemical interaction between AuNPs and -OH of rGO in GG1PD, Their morphologies, structures, electrochemical properties, and gene nanobiosensing performances were characterized and evaluated. AuNPs/GG2PD-based probe displayed the best excellent structural stability, lowest mobility on solid surface with the increasing charge resistance, widest linear range (1.1 × 10-6 - 1 × 10-18), and the lowest limit of detection (1.87 × 10-19 M) in comparison with both AuNPs/GG1PD-based and AuNPs/GG3PD-based prob...Continue Reading

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