Layer-by-layer electrochemical biosensor with aptamer-appended active polyelectrolyte multilayer for sensitive protein determination

Biosensors & Bioelectronics
Yan DuShaojun Dong

Abstract

Herein, we report two simple label-free electrochemical aptasensors for protein detection using layer-by-layer (LBL) self-assembled multilayers with ferrocene-appended poly(ethyleneimine) (Fc-PEI), carbon nanotubes (CNTs) and aptamer. In one sensing strategy, the Fc-PEI, CNTs and DNA aptamer are LBL assembled on the electrode surface via electrostatic interaction. In the presence of target, the aptamer on the outermost layer of the LBL self-assembled multilayer would catch the target on the electrode interface, which makes a barrier for electrons and inhibits the electro-transfer, resulting in the decreased DPV signals of Fc-PEI. Using this strategy, a wide detection range (0.3-165 ng ml(-1)) for model target thrombin is obtained, with a low detection limit of 0.14 ng ml(-1). In the similar sensing strategy for detection of lysozyme, a wide detection range (0.2 ng ml(-1) to 1.66 microg ml(-1)) and a low detection limit (0.17 ng ml(-1)) are obtained. These results prove that the LBL sensing strategies developed possess sensitivity, selectivity, stability and generality.

Citations

Feb 23, 2011·International Journal of Molecular Sciences·Linghua MengLei Zheng
Feb 7, 2013·Journal of Computational Chemistry·Sandra C C NunesA A C C Pais
Jun 7, 2011·Bioprocess and Biosystems Engineering·Tao SunXianxue Gan
Mar 18, 2011·Faraday Discussions·Anthony E G Cass, Yangyang Zhang
Dec 6, 2013·Materials·Shigehiro Takahashi, Jun-Ichi Anzai
Jun 11, 2020·Sensors·Ivana Škugor RončevićMarijo Buzuk
May 29, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jared L DeLucaDavid W Schmidtke
Nov 18, 2020·International Journal of Biological Macromolecules·Niyati H MudliarPrabhat K Singh
May 16, 2013·Journal of the American Chemical Society·Ramy A GhostineJoseph B Schlenoff

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