Layer-by-layer electrochemical biosensors configuring xanthine oxidase and carbon nanotubes/graphene complexes for hypoxanthine and uric acid in human serum solutions

Biosensors & Bioelectronics
Yunpei SiHye Jin Lee

Abstract

A selective biosensing platform for the determination of hypoxanthine (Hx) and uric acid (UA) concentrations in both buffer and human serum sample solutions was developed. The biosensor features the layer-by-layer (LbL) self-assembly of negatively charged xanthine oxidase (XOD) and positively charged poly(diallyldimethyl ammonium chloride) (PDDA) wrapped oxidized multi-walled carbon nanotubes and graphene (CNTs-G) complexes (PDDA-CNTs-G) on screen printed carbon electrode (SPCE) surfaces. Catalytic responses of the XOD modified biosensor with the chosen optimum number of layers for LbL assembly on SPCE towards Hx in buffer solutions were first investigated using both cyclic and square wave voltammetries. The peak current at around 0.08 V (vs. Ag/AgCl) associated with the production of UA increased as a function of the Hx concentration due to the surface selective catalytic reaction of XOD and Hx. A linear dynamic range of 5-50 µM Hx with a detection limit of 4.40 µM was obtained and the sensor was further applied to the analysis of Hx in normal human serum solutions in addition to myocardial infarction (MI) patient serum sample solutions from a local hospital. Since untreated serum solutions contain a certain amount of UA, a XO...Continue Reading

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Citations

May 31, 2019·Physical Chemistry Chemical Physics : PCCP·Bianca T KitagakiRaphael Nagao
Apr 30, 2020·Journal of Food Biochemistry·Arshad MehmoodMuhammad Nadeem
Dec 21, 2018·Micromachines·Aihua JingChenhuan Jing
Apr 21, 2021·Nanoscale Research Letters·Ahmet AykaçMustafa Erol
May 1, 2021·3 Biotech·Nirmal Kant SharmaTek Chand Bhalla
Jun 30, 2021·ACS Sensors·Kelcilene B R TeodoroDaniel S Correa
Oct 15, 2020·Chemical Reviews·Hui ChenShelley D Minteer

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