Laying the Foundation for Crassulacean Acid Metabolism (CAM) Biodesign: Expression of the C4 Metabolism Cycle Genes of CAM in Arabidopsis

Frontiers in Plant Science
Sung Don LimJ C Cushman

Abstract

Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that exploits a temporal CO2 pump with nocturnal CO2 uptake and concentration to reduce photorespiration, improve water-use efficiency (WUE), and optimize the adaptability of plants to hotter and drier climates. Introducing the CAM photosynthetic machinery into C3 (or C4) photosynthesis plants (CAM Biodesign) represents a potentially breakthrough strategy for improving WUE while maintaining high productivity. To optimize the success of CAM Biodesign approaches, the functional analysis of individual C4 metabolism cycle genes is necessary to identify the essential genes for robust CAM pathway introduction. Here, we isolated and analyzed the subcellular localizations of 13 enzymes and regulatory proteins of the C4 metabolism cycle of CAM from the common ice plant in stably transformed Arabidopsis thaliana. Six components of the carboxylation module were analyzed including beta-carbonic anhydrase (McBCA2), phosphoenolpyruvate carboxylase (McPEPC1), phosphoenolpyruvate carboxylase kinase (McPPCK1), NAD-dependent malate dehydrogenase (McNAD-MDH1, McNAD-MDH2), and NADP-dependent malate dehydrogenase (McNADP-MDH1). In addition, seven components of the decarboxyl...Continue Reading

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Citations

May 16, 2019·Journal of Experimental Botany·Sarah C DavisAlberto Búrquez
Jan 17, 2020·The Plant Journal : for Cell and Molecular Biology·Methawi Chomthong, Howard Griffiths
Apr 28, 2020·The Plant Journal : for Cell and Molecular Biology·Sung Don LimJohn C Cushman
Nov 30, 2019·Journal of Experimental Botany·Kevin R HultineDavid G Williams
Jul 10, 2019·Current Opinion in Plant Biology·Nicholas A NiechayevJohn C Cushman
Oct 13, 2021·The New Phytologist·Klaus Winter, J Andrew C Smith

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Methods Mentioned

BETA
PCR
transgenic
RNA-seq
confocal microscopy

Software Mentioned

ImageJ
mLoc
trans
FUEL
EvidentialGene
SOAPdenovo

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