LC-ESI-MS determination and pharmacokinetics of adrafinil in rats

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
R Nageswara RaoSachin B Agawane

Abstract

A highly sensitive and specific liquid chromatography/tandem mass spectrometric (LC-MS/MS) method for investigating the pharmacokinetics of adrafinil in rats was developed. Rat serum pretreated by solid-phase extraction (SPE) was analyzed by LC-MS/MS with an electrospray ionization (ESI) interface. The mobile phase consisted of acetonitrile:water:acetic acid (35:65:0.1, v/v/v) in an isocratic elution mode pumped at 1.0 ml/min. The analytical column (250 mm x 4.6 mm i.d.) was packed with Kromasil C(18) material (5.0 microm). The standard curve was linear from 16.5 to 5000 ng/ml. The assay was specific, accurate (R.S.D.<2.6%), precise and reproducible (within- and between-day precisions R.S.D. <7.0% and <9.0%, respectively). Adrafinil in rat serum was stable over three freeze-thaw cycles at ambient temperature for 6 h. The method had a lower limit of quantitation of 16.5 ng/ml, which offered high sensitivity for the determination of adrafinil in serum. The method was successfully applied to pharmacokinetic studies of adrafinil after an oral administration to rats.

References

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Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Steven H Gorman
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Apr 19, 2005·Rapid Communications in Mass Spectrometry : RCM·Andrew R McKinneyAllen M Stenhouse

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Citations

Apr 12, 2011·Analytical and Bioanalytical Chemistry·K DeventerP Van Eenoo
Jun 14, 2016·Chemical Communications : Chem Comm·Mehmet Gokhan CaglayanPavel Anzenbacher
Apr 29, 2009·Rapid Communications in Mass Spectrometry : RCM·Jianghai LuGangfeng Ouyang

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