LC method for the direct and simultaneous determination of four major furan derivatives in coffee grounds and brews

Journal of Separation Science
Abdullatif Albouchi, Michael Murkovic

Abstract

The simultaneous quantification of two potential genotoxic hydroxymethyl furan derivatives in coffee (furfuryl alcohol and 5-hydroxymethylfurfural) alongside their carboxylic acid derivatives (2-furoic acid and 5-hydroxymethyl furoic acid, respectively) was carried out. Their extraction from ground roasted coffee using sonication, simple shaking or heat-assisted shaking lead to similar results. A minimum of 97.3% of the four furan derivatives were extracted during the first extraction cycle using water, whereas methanol showed considerably lower extraction efficiency. A simple high-performance liquid chromatography method coupled with diode array detection was developed for the simultaneous determination and was applied to roasted coffee extracts or brews. No sample pre-treatment except for centrifugation was needed. The diode array detector was used to assess the purity of the peaks of interest in analyzed samples against authentic standards. The linearity according to Mandel, accuracy (recovery ≥ 89.9%) and precision (inter- and intraday relative standard deviation ≤ 4.5%) were checked. The values for the limit of detection and quantification ranged within 0.11-0.76 and 0.35-2.55 μg/mL, respectively. Filtered and espresso bre...Continue Reading

References

Aug 19, 2006·Molecular Nutrition & Food Research·Michael Murkovic, Nicole Pichler
Mar 16, 2007·Molecular Nutrition & Food Research·Michael Murkovic, Maria-Anna Bornik
Jul 7, 2009·Journal of Agricultural and Food Chemistry·Joon-Kwan Moon, Takayuki Shibamoto
Nov 18, 2017·Food Chemistry·Abdullatif Albouchi, Michael Murkovic

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