LC-MS determination of bioactive molecules based upon stable isotope-coded derivatization method

Journal of Pharmaceutical and Biomedical Analysis
Toshimasa Toyo'oka

Abstract

Liquid chromatography (LC) coupled with mass spectrometry (MS) has been widely used for the analyses of various molecules in many research fields. The electrospray ionization of MS has contributed to the advancement of the LC-MS and LC-MS/MS methods. However, the detection sensitivity is not always sufficient in biological samples, in spite of the highly sensitive ionization method. To increase the sensitivity, chemical derivatization, providing ionization enhancement and avoiding the matrix effect, is effective for various functional groups in the target molecules. However, the accuracy and precision by the determination is sometimes insufficient, especially in complex matrices. In such a case, stable isotope-labeled analogs are often used as the internal standards for the determination of the analytes. When the target compound in samples is limited, a high accuracy and precision is usually obtained by the isotope dilution method. However, the use of individual isotope standards is very difficult for the analyses of multiple molecules in complex matrices. Instead of the use of an isotope analog of the analytes, the differential isotope labeling method based upon chemical derivatization (stable isotope-coded derivatization) (IC...Continue Reading

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