LC-MS metabolomics of polar compounds

Bioanalysis
David RojoFrancisco J Rupérez

Abstract

The metabolome is the complete set of small molecules coming from protein activity (anabolism and catabolism) in living systems. They have a broad range of chemical structures and physicochemical properties and therefore different analytical methodologies are necessary. Highly polar metabolites, such as sugars and most amino acids are not retained by conventional reversed-phase LC columns. Without sufficient retention, coelution may result in identification problems while the detection of compounds by MS at low concentrations may also be problematic due to ion suppression. In order to retain compounds based on their hydrophilicity, polar stationary phases and hydrophilic-interaction LC provide a complementary tool to reversed-phase LC for untargeted comprehensive metabolite fingerprinting. However, robustness of the methods is still limiting their applications. This review focuses on sample pretreatment, stationary phases, analytical methods and applications for polar compound analysis in biological matrices.

References

Sep 27, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Helena IdborgSven P Jacobsson
Nov 2, 2007·Analytical Chemistry·Simon CubbonJane Thomas-Oates
Feb 26, 2008·Journal of Chromatography. a·Tohru IkegamiNobuo Tanaka
Mar 19, 2008·Journal of Separation Science·Haleem J IssaqTimothy D Veenstra
Apr 11, 2008·Journal of Separation Science·Hien P Nguyen, Kevin A Schug
Apr 23, 2008·Journal of Separation Science·Yunsheng Hsieh
May 8, 2008·Journal of Separation Science·Bieke DejaegherYvan Vander Heyden
Jan 28, 2009·Journal of Agricultural and Food Chemistry·Hiroo YoshidaHiroshi Miyano
Jun 2, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Kazutaka IkedaRyo Taguchi
Jun 27, 2009·Mass Spectrometry Reviews·Simon CubbonJane Thomas-Oates
Jul 2, 2009·Journal of Separation Science·Edward GoucherSue Jickells
Nov 21, 2009·Phytochemical Analysis : PCA·J William Allwood, Royston Goodacre
Feb 27, 2010·Journal of Separation Science·Konstantina SpagouGeorgios Theodoridis
Dec 15, 2010·Analytical Chemistry·Konstantina SpagouElizabeth J Want
Dec 15, 2010·PLoS Neglected Tropical Diseases·Ruben t'KindtSaskia Decuypere
May 31, 2011·Journal of Chromatography. a·José BernalSusanne K Wiedmer
Jun 7, 2011·Journal of Chromatography. a·Oswaldo Hernández-HernándezM Luz Sanz
Jul 9, 2011·Journal of Chromatography. a·Yong Guo, Sheetal Gaiki
Aug 2, 2011·Journal of Chromatography. a·Ngoc Phuoc DinhKnut Irgum
Oct 6, 2011·Journal of Separation Science·Gary J Patti

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Citations

Jun 19, 2013·Experimental Eye Research·Igor A Butovich
Dec 18, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Mukesh VermaJohn P A Ioannidis
Feb 13, 2014·Analytical Chemistry·Ewelina P DutkiewiczPawel L Urban
Apr 12, 2014·Analytical and Bioanalytical Chemistry·Gisele A B CanutoÁngeles López-Gonzálvez
May 9, 2014·Bioanalysis·Rawi Ramautar, Gerhardus J de Jong
Jan 16, 2016·Analytical and Bioanalytical Chemistry·Juan Carlos García-CañaverasAgustín Lahoz
Feb 26, 2013·Electrophoresis·Zacarías LeónAgustín Lahoz
Jul 17, 2015·Electrophoresis·Miguel Ángel García-SevillanoJosé Luis Gómez-Ariza
Jul 28, 2016·Journal of Pharmaceutical and Biomedical Analysis·Carolina Gonzalez-RianoCoral Barbas
Jul 11, 2019·Metabolomics : Official Journal of the Metabolomic Society·Ho-Joon LeeCostas A Lyssiotis
Jul 25, 2019·International Journal of Molecular Sciences·Aneta ZebrowskaMonika Pietrowska
May 8, 2019·Current Rheumatology Reports·Salah Ali A ShowiheenIndira Prasadam
May 21, 2019·Electrophoresis·Marlien van MeverRawi Ramautar
Sep 4, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marina Creydt, Markus Fischer

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Methods Mentioned

BETA
NMR
ionic exchange

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