LC-MS-MS method for simultaneous determination of THCCOOH and THCCOOH-glucuronide in urine: Application to workplace confirmation tests

Forensic Science International : Synergy
M FelliM Chiarotti

Abstract

Cannabis is the one of the most frequently detected drug in workplace drug testing and in cases of driving under influence of drugs, and there is a greater demand for sensitive, rapid and reliable methods for confirming the presence of this drug in biological samples. This paper describes an LC-MS-MS procedure for direct analysis of cTHC and cTHC-glucuronide in urine, without hydrolysis of the samples or derivatisation. The sample preparation is very simple and consist only in a dilution of urine with methanol 1:1 (v/v) after adding the deuterated internal standard (cTHC-d3); then 10μl of the final solution is injected in LC-MS-MS. Chromatographic separation was performed using a reversed-phase column and gradient elution with 0.01% formic acid/acetonitrile/methanol and two MS-MS transitions for each substance were monitored. The method was fully validated and proved to be accurate (RSD <15%), precise (intra-day CV <10%) and sensitive with a limit of detection (LOD) of 5ng/ml for both the compounds studied. Linearity was tested in the range 5-1000ng/ml and the values of R(2) resulted >0.99. The developed method was applied to several authentic samples of urine which tested positive in the immunoassay screening and 98% of them w...Continue Reading

References

Sep 9, 2000·Forensic Science International : Synergy·W WeinmannA Bromberger
Aug 23, 2001·Forensic Science International : Synergy·A G Verstraete, A Pierce
Apr 22, 2006·Therapeutic Drug Monitoring·Frank Musshoff, Burkhard Madea
Jun 20, 2006·Forensic Science International : Synergy·Frank T PetersFrank Musshoff

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Citations

Oct 30, 2012·Analytical and Bioanalytical Chemistry·N FerreirósG Geisslinger
Dec 3, 2013·Analytical and Bioanalytical Chemistry·Nathalie A DesrosiersMarilyn A Huestis
Jan 1, 2016·Journal of Chromatography. a·M Concepción Cela-PérezM Victoria González-Rodríguez
Nov 13, 2013·Drug Testing and Analysis·Natalia BattistaMauro Maccarrone
Jan 29, 2014·Journal of Pharmaceutical and Biomedical Analysis·Camilla MontesanoRoberta Curini
Jul 10, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Karl B ScheidweilerMarilyn A Huestis
Nov 14, 2014·Analytical and Bioanalytical Chemistry·Stefan W ToennesAlexander Paulke
Jun 3, 2004·Forensic Science International : Synergy·Olaf H Drummer
Aug 16, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoru DongYan Jiang
Feb 6, 2021·Journal of Analytical Toxicology·Aykut Kul, Olcay Sagirli

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