LC-MS Untargeted Metabolomics To Explain the Signal Metabolites Inducing Browning in Fresh-Cut Lettuce

Journal of Agricultural and Food Chemistry
Carlos J GarcíaFrancisco A Tomas-Barberan

Abstract

Enzymatic browning is one of the main causes of quality loss in lettuce as a prepared and ready-to-eat cut salad. An untargeted metabolomics approach using UPLC-ESI-QTOF-MS was performed to explain the wound response of lettuce after cutting and to identify the metabolites responsible of browning. Two cultivars of Romaine lettuce with different browning susceptibilities were studied at short time intervals after cutting. From the total 5975 entities obtained from the raw data after alignment, filtration reduced the number of features to 2959, and the statistical analysis found that only 1132 entities were significantly different. Principal component analysis (PCA) clearly showed that these samples grouped according to cultivar and time after cutting. From those, only 15 metabolites belonging to lysophospholipids, oxylipin/jasmonate metabolites, and phenolic compounds were able to explain the browning process. These selected metabolites showed different trends after cutting; some decreased rapidly, others increased but decreased thereafter, whereas others increased during the whole period of storage. In general, the fast-browning cultivar showed a faster wound response and a higher raw intensity of some key metabolites than the ...Continue Reading

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Citations

Feb 23, 2018·Protoplasma·Elena T Iakimova, Ernst J Woltering
Jun 10, 2018·Journal of the Science of Food and Agriculture·Luiz F Coelho JúniorAdriano N Simoes
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Rob van TreurenRic C H de Vos
Oct 6, 2020·Postharvest Biology and Technology·Annabelle DamerumGail Taylor
Sep 17, 2020·Journal of Agricultural and Food Chemistry·Keabetswe MasikeMaria A Stander
May 2, 2019·Journal of Agricultural and Food Chemistry·Carlos J GarcíaFrancisco A Tomás-Barberán
May 1, 2020·Food Chemistry·Aline G CunhaMaria Raquel A de Miranda

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