LC-MS/MS-based serum proteomics for identification of candidate biomarkers for hepatocellular carcinoma

Proteomics
Tsung-Heng TsaiHabtom W Ressom

Abstract

Associating changes in protein levels with the onset of cancer has been widely investigated to identify clinically relevant diagnostic biomarkers. In the present study, we analyzed sera from 205 patients recruited in the United States and Egypt for biomarker discovery using label-free proteomic analysis by LC-MS/MS. We performed untargeted proteomic analysis of sera to identify candidate proteins with statistically significant differences between hepatocellular carcinoma (HCC) and patients with liver cirrhosis. We further evaluated the significance of 101 proteins in sera from the same 205 patients through targeted quantitation by MRM on a triple quadrupole mass spectrometer. This led to the identification of 21 candidate protein biomarkers that were significantly altered in both the United States and Egyptian cohorts. Among the 21 candidates, ten were previously reported as HCC-associated proteins (eight exhibiting consistent trends with our observation), whereas 11 are new candidates discovered by this study. Pathway analysis based on the significant proteins reveals upregulation of the complement and coagulation cascades pathway and downregulation of the antigen processing and presentation pathway in HCC cases versus patient...Continue Reading

References

Sep 30, 1994·Statistics in Medicine·M N ChangJ F Heyse
Apr 1, 1996·Journal of Gastroenterology and Hepatology·Y SuzukiH Asakura
Aug 17, 2001·European Journal of Gastroenterology & Hepatology·M TakeuchiT Hayakawa
Mar 11, 2003·Technology in Cancer Research & Treatment·Rene C KriegEmanuel F Petricoin
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
Sep 4, 2003·Genome Research·Paul D ThomasApurva Narechania
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Feb 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shu-Chen ChuHui-Ling Chiou
Jan 26, 2005·Human Pathology·Yun Kyung KangWoo Ho Kim
Feb 4, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Valérie ParadisPierre Bedossa
Nov 4, 2006·Proteomics·Shen HuDavid T Wong
Aug 2, 2007·Analytical and Bioanalytical Chemistry·Marcus BantscheffBernhard Kuster
Jan 29, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Noah T ZinkinNezam H Afdhal
Mar 12, 2008·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Eldad S Bialecki, Adrian M Di Bisceglie
Aug 13, 2008·Nature Clinical Practice. Oncology·Vathany Kulasingam, Eleftherios P Diamandis
Aug 22, 2008·Journal of Proteome Research·Raghothama ChaerkadyPaul J Thuluvath
Oct 16, 2008·Molecular Systems Biology·Vinzenz LangeRuedi Aebersold
Oct 23, 2008·Nucleic Acids Research·Lars J JensenChristian von Mering
Nov 8, 2008·Nucleic Acids Research·T S Keshava PrasadAkhilesh Pandey
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jun 25, 2009·Chemical Society Reviews·William J Griffiths, Yuqin Wang
Nov 3, 2009·Nucleic Acids Research·Minoru KanehisaMika Hirakawa
Nov 13, 2010·Nucleic Acids Research·Chris StarkMike Tyers
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Feb 26, 2011·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayDonald Maxwell Parkin
Oct 14, 2011·The New England Journal of Medicine·Hashem B El-Serag
May 5, 2012·PloS One·Illés J FarkasTamás Korcsmáros
Jul 10, 2012·Analytical and Bioanalytical Chemistry·Marcus BantscheffBernhard Kuster
Oct 20, 2012·Journal of Proteome Research·Jun Feng XiaoHabtom W Ressom
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Jan 25, 2013·Nature Reviews. Cancer·Alla ArzumanyanMark A Feitelson

❮ Previous
Next ❯

Citations

Oct 21, 2015·Drug Discovery Today·Matheus H DiasLeo K Iwai
Jun 2, 2016·IEEE Journal of Biomedical and Health Informatics·Minkun WangHabtom W Ressom
Sep 7, 2016·Methods : a Companion to Methods in Enzymology·Yiming ZuoHabtom W Ressom
Oct 30, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Abdel Hady A Abdel WahabZakaria Y Abd Elmageed
Jun 12, 2018·Mass Spectrometry Reviews·Iwona BelczackaMaria Frantzi
Jan 9, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Ali NehmeKazem Zibara
Jan 16, 2019·Molecular Carcinogenesis·Yuhui TanJianyong Xiao
Jan 5, 2020·Nature Reviews. Gastroenterology & Hepatology·Bin ChenXin Chen
Dec 13, 2018·Virusdisease·Kruti DalalAruna Shankarkumar
Nov 18, 2015·World Journal of Gastroenterology : WJG·René FahrnerFalk Rauchfuß
Oct 3, 2018·Journal of Ovarian Research·Agata SwiatlyZenon J Kokot
May 31, 2019·Expert Review of Proteomics·Kwang Hoe KimJong Shin Yoo
Aug 28, 2020·Statistics in Medicine·Gajendra K VishwakarmaBenoit Liquet
Jan 17, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·K S JangJ S Kim
Mar 6, 2021·Proteomics·Vivian Weiwen XueWilliam Chi Shing Cho
Apr 6, 2021·Open Life Sciences·Junwei ShuShuangjiang Chen
May 21, 2021·Network Modeling and Analysis in Health Informatics and Bioinformatics·Muaaz Gul AwanFahad Saeed
Jul 29, 2021·Movement Disorders : Official Journal of the Movement Disorder Society·Yan V SunHyder A Jinnah
Jan 21, 2017·Analytical Chemistry·Stefan GaunitzMilos V Novotny

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.