LC-MS/MS determination of FTY720 and FTY720-phosphate in murine intracellular compartments and human plasma

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
N FerreirósG Geisslinger

Abstract

A new analytical method for the quantitation of the orally active immunomodulatory drug FTY720 and its phosphate derivative in human plasma and murine subcellular compartments has been developed. The samples undergo a liquid-liquid extraction process before they are injected into a liquid chromatographic system coupled to a tandem mass spectrometer operating in positive ion mode. The quantitation is based on the analysis of two multiple reaction monitoring transitions per drug. The recovery of the analytical process is around 80% for all analytes. Intra- and interday precision and accuracy, as relative standard deviation and relative error, respectively, are lower than 12.5% in all cases. No important matrix effects were observed. The lower limits of quantitation for the analysed substances were 0.875 ng/mL and 2 ng/mL for FTY720 and FTY720-phosphate, respectively. Since no deuterated derivatives of the analytes were commercially available, the developed method was applied for quantifying the studied compounds using C17-sphingosine and C-17-sphingosine-1-phosphate as internal standards, in subcellular compartments of murine splenocytes. This method could be applied in the future for monitoring purposes in multiple sclerosis pat...Continue Reading

References

Feb 25, 2000·Therapeutic Drug Monitoring·K L Napoli
Dec 1, 2004·Therapeutic Drug Monitoring·John M KovarikAlan J Slade

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Citations

Aug 19, 2015·Journal of Pharmaceutical and Biomedical Analysis·Prinesh N PatelR Srinivas
Apr 12, 2015·The Journal of Surgical Research·Hung-Jen ShihChun-Jen Huang
Oct 11, 2017·Biomedical Chromatography : BMC·Ranjeet Prasad DashRana Rais
Nov 13, 2015·Scientific Reports·Zhaochuan LiuWeirong Chen
Jan 7, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hesham SalemDina Heshmat

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