LC-MS/MS glycomics of idiopathic rapid eye movement sleep behavior disorder

Electrophoresis
Xue DongYehia Mechref

Abstract

Idiopathic REM sleep behavior disorder (iRBD) is now considered a prodromal stage of an α-synucleinopathy-related to neurodegenerative disease such as Parkinson's diseases. Emerging evidence has shown that post-translational modification or glycosylation are implicated in dynamic disease mechanisms and the onset of many pathological conditions. We hypothesized that the characterization of the glycosylation pattern of patients with RBD would be of great value to understand the pathophysiology and underlying mechanisms and represent potentially useful biomarkers for disease-associated molecular changes. To test this hypothesis, we assessed the serum glycome of patients with RBD and compared to that of healthy controls. NanoRPLC-MS was used to generate quantitative N-glycan profiles while high-temperature PGC-LC-MS platform was employed to generate quantitative isomeric N-glycan profiles. By analyzing permethylated glycans derived from human blood sera on C18-LC-MS/MS, we identified 59 N-glycan structures in healthy (control) cohort, 56 N-glycans in RBD cohort. Sixteen N-glycans structures were found to be significantly altered in the RBD cohort (p < 0.05). N-glycans with the composition of HexNAc4 Hex5 Fuc1 , HexNAc5 Hex5 , and H...Continue Reading

References

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Citations

Jun 24, 2021·Mass Spectrometry Reviews·Wenjing PengYehia Mechref
Jun 3, 2020·Journal of Proteome Research·Xue DongYehia Mechref
Sep 11, 2021·Electrophoresis·Kaitlyn B DonohooYehia Mechref
Feb 8, 2022·Movement Disorders : Official Journal of the Movement Disorder Society·Ranjani Ganapathy SPetr Dušek

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